Ursolic acid inhibits the proliferation of human ovarian cancer stem-like cells through epithelial-mesenchymal transition

Oncology Reports
Jie ZhangCong Qi

Abstract

Ovarian cancer is the most frequent cause of cancer-related death among all gynecological cancers. Increasing evidence suggests that human ovarian cancer stem-like cells could be enriched under serum-free culture conditions. In the present study, SKOV3 ovarian epithelial cancer cells were cultured for sphere cells. Ursolic acid (UA) with triterpenoid compounds exist widely in food, medicinal herbs and other plants. Evidence shows that UA has anticancer activities in human ovarian cancer cells, but he role of UA in ovarian cancer stem cells (CSCs) remains unknown. The aim of the present study was to investigate the anticancer effects of UA in combination with cisplatin in ovarian CSCs (in vitro and in vivo), along with the molecular mechanism of action. Treatment with UA at various concentrations was examined in combination with cisplatin in human ovarian CSCs. MTT assay and flow cytometry were used for cell viability and apoptosis analysis, and qRT-PCR for stem cell markers and epithelial-mesenchymal transition (EMT) markers for mRNA expression. Transwell assay was employed to observe the migration and invasion of SKOV3 cells and SKOV3 sphere cells after treatment. Moreover, athymic BALB/c-nu nude mice were injected with SKOV3 ...Continue Reading

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Citations

Jan 7, 2016·Journal of Cancer Prevention·Eun-Yi Ko, Aree Moon
Feb 15, 2020·Natural Product Reports·Robert A Hill, Joseph D Connolly
Mar 5, 2019·Journal of Asian Natural Products Research·Yan-Qiu MengXiao-Xiao Li
Dec 14, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Yaohan WangPeitong Zhang
Dec 6, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chia-Chieh WuYao-Li Chen
Feb 4, 2021·International Journal of Molecular Sciences·Marta WoźniakSiddarth Agrawal
Dec 10, 2020·Cancers·Xiaona LiuLei Liu

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