Us3 and Us9 proteins contribute to the stromal invasion of bovine herpesvirus 1 in the respiratory mucosa

The Journal of General Virology
Jing ZhaoHans J Nauwynck

Abstract

Bovine herpesvirus 1 (BHV-1) infection may lead to conjunctivitis, upper respiratory tract problems, pneumonia, genital disorders and abortion. BHV-1 is able to spread quickly in a plaque-wise manner and invade by breaching the basement membrane (BM) barrier in the respiratory mucosa. BHV-1 Us3, a serine/threonine kinase, induces a dramatic cytoskeletal reorganization and BHV-1 Us9, a tail-anchored membrane protein, is required for axonal transport of viruses in neurons. In this study, we investigated the role of Us3 and Us9 during BHV-1 infection in the respiratory mucosa. First, we constructed and characterized BHV-1 Us3 null, Us9 null and revertant viruses. Then, we analysed the viral replication and plaque size (latitude) in Madin-Darby bovine kidney (MDBK) cells and the respiratory mucosa as well as viral penetration depth underneath the BM of the respiratory mucosa when inoculated with these recombinant viruses. Knockout of Us3 resulted in a 1 log10 reduction in viral titre and plaque size (latitude) in MDBK cells and the trachea mucosa. There were no defects in the cell-to-cell spread observed for BHV-1 Us9 null virus. Both BHV-1 Us3 null and Us9 null viruses showed a significant reduction of plaque penetration underneat...Continue Reading

References

Jan 1, 1995·Advances in Virus Research·S K TikooL A Babiuk
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·R LeopardiB Roizman
Jan 1, 1997·British Medical Bulletin·L S YoungA G Eliopoulos
Jun 18, 2002·Microbes and Infection·Takayuki MurataYukihiro Nishiyama
Jun 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Luca Benetti, Bernard Roizman
Jan 30, 2007·Veterinary Research·Benoît MuylkensEtienne Thiry
Aug 10, 2007·Journal of Virology·M G LymanL W Enquist
Aug 21, 2009·The Journal of General Virology·Shaunivan L LabiukSylvia van Drunen Littel-van den Hurk
Dec 18, 2009·The Journal of General Virology·Shaunivan L LabiukSylvia van Drunen Littel-van den Hurk
Mar 4, 2010·Veterinary Microbiology·Agnieszka BrzozowskaKrystyna Bieńkowska-Szewczyk
Jul 14, 2010·The Veterinary Clinics of North America. Food Animal Practice·Clinton Jones, Shafiqul Chowdhury
Aug 3, 2010·Methods in Molecular Biology·B Karsten TischerNikolaus Osterrieder
Oct 15, 2010·The Journal of General Virology·M J Deruelle, H W Favoreel
Jun 15, 2012·International Journal of Cancer. Journal International Du Cancer·T S SalamehI G Camarillo
Aug 29, 2016·Archives of Virology·Sita Awasthi, Harvey M Friedman

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.