PMID: 9546181Apr 18, 1998Paper

Use of 13C nuclear magnetic resonance to assess fossil fuel biodegradation: fate of [1-13C]acenaphthene in creosote polycyclic aromatic compound mixtures degraded by bacteria

Applied and Environmental Microbiology
S A SelifonovP G Hatcher

Abstract

[1-13C]acenaphthene, a tracer compound with a nuclear magnetic resonance (NMR)-active nucleus at the C-1 position, has been employed in conjunction with a standard broad-band-decoupled 13C-NMR spectroscopy technique to study the biodegradation of acenaphthene by various bacterial cultures degrading aromatic hydrocarbons of creosote. Site-specific labeling at the benzylic position of acenaphthene allows 13C-NMR detection of chemical changes due to initial oxidations catalyzed by bacterial enzymes of aromatic hydrocarbon catabolism. Biodegradation of [1-13C]acenaphthene in the presence of naphthalene or creosote polycyclic aromatic compounds (PACs) was examined with an undefined mixed bacterial culture (established by enrichment on creosote PACs) and with isolates of individual naphthalene- and phenanthrene-degrading strains from this culture. From 13C-NMR spectra of extractable materials obtained in time course biodegradation experiments under optimized conditions, a number of signals were assigned to accumulated products such as 1-acenaphthenol, 1-acenaphthenone, acenaphthene-1,2-diol and naphthalene 1,8-dicarboxylic acid, formed by benzylic oxidation of acenaphthene and subsequent reactions. Limited degradation of acenaphthene...Continue Reading

References

Nov 1, 1992·Applied and Environmental Microbiology·J V PothuluriC E Cerniglia
Jul 1, 1984·Applied and Environmental Microbiology·M J Schocken, D T Gibson
Oct 1, 1995·Applied and Environmental Microbiology·M GrifollP J Chapman
Jul 1, 1994·Applied and Environmental Microbiology·M GrifollP J Chapman
Jun 1, 1995·Environmental Health Perspectives·P J ChapmanS Selifonov
Feb 1, 1996·Applied and Environmental Microbiology·S A SelifonovP J Chapman

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Citations

Jan 30, 2015·Magnetic Resonance in Chemistry : MRC·Caroline M Preston
Sep 20, 2005·Archives of Environmental Contamination and Toxicology·J K C NiemanJ E McLean
Dec 3, 2010·Environmental Science & Technology·Carsten JobeliusChristian Zwiener
Feb 3, 2007·Environmental Science & Technology·J Karl C NiemanRonald C Sims

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