Use of 13C nuclear magnetic resonance spectroscopy for the evaluation of hepatic drug-metabolizing enzyme activity in perfused mouse livers

Journal of Biochemical and Biophysical Methods
M A PassI C Smith

Abstract

Experiments were performed to determine whether the activity of hepatic drug-metabolizing enzymes could be estimated in intact livers by 13C NMR spectroscopy. 13C-labelled aminopyrine was administered to isolated perfused mouse livers and the rate of decline in the NMR signal arising from the drug was used to estimate the half-life of aminopyrine. The technique was sufficiently sensitive to discriminate between the enzyme activities in normal livers and in livers whose enzyme activity was enhanced by treatment with sodium phenobarbitone. It was also demonstrated that demethylation of aminopyrine was not impaired in perfused livers from mice infected with Plasmodium berghei.

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Citations

Apr 1, 1990·European Journal of Drug Metabolism and Pharmacokinetics·J D BellN E Preece
Sep 1, 1992·Biochimie·M C Malet-Martino, R Martino
Feb 1, 1989·Clinical Biochemistry·I C Smith
Feb 1, 1985·Quarterly Reviews of Biophysics·R DeslauriersI C Smith
Jun 1, 1989·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M C Malet-Martino, R Martino
Jan 1, 1989·The Journal of Surgical Research·A W BoddieL Dennis
Jan 1, 1988·Medicinal Research Reviews·N E MacKenzie, P R Gooley
Jan 11, 2002·Journal of Advanced Nursing·Lynne S SchillingKathleen A Knafl
Apr 1, 1996·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J C StreetM A Jarema

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