PMID: 2123176Sep 1, 1990Paper

Use of 13C substrates for metabolic studies in exercise: methodological considerations

Journal of Applied Physiology
F PéronnetC Hillaire-Marcel

Abstract

The purpose of this study is to outline a common mistake made when the rate of oxidation of exogenous substrates during prolonged exercise is computed using 13C naturally labeled substrates. The equation proposed and commonly used in the computation does not take into account that exercise and/or exogenous substrate ingestion modifies the composition of the mixture of endogenous substrates oxidized and, consequently, the isotopic composition of CO2 arising from oxidation of endogenous substrates. The recovery of 13C and the amount of exogenous substrate oxidized are thus overestimated. An adequate procedure for the computation of exogenous substrate oxidation taking into account changes in isotopic composition of CO2 arising from oxidation of endogenous substrates is suggested. Results from a pilot experiment (4 subjects) using this procedure indicate that over 2 h of exercise (66% of maximal O2 uptake), with ingestion of 60 g of glucose, 39 +/- 4 g of glucose were oxidized. Estimates made without taking into account changes in isotopic composition of CO2 arising from oxidation of endogenous substrates range between 70 +/- 8 and 44 +/- 3 g depending on 1) the isotopic composition of exogenous glucose and 2) the isotopic composi...Continue Reading

Citations

Aug 4, 1998·Journal of Applied Physiology·F PéronnetD Massicotte
Jun 30, 2001·The British Journal of Nutrition·N FolchC Hillaire-Marcel
Feb 25, 2005·American Journal of Physiology. Endocrinology and Metabolism·L MoseleyA E Jeukendrup
Apr 19, 2008·Journal of Applied Physiology·Michael C Riddell
Jul 24, 2010·Journal of Applied Physiology·Denis P BlondinFrançois Haman
Jun 18, 2019·Medicine and Science in Sports and Exercise·John P O'HaraRoderick F G J King
Jan 1, 1993·European Journal of Applied Physiology and Occupational Physiology·D MassicotteC Hillaire-Marcel
Jan 1, 1996·European Journal of Applied Physiology and Occupational Physiology·G P LeeseM J Rennie
Jan 1, 1994·European Journal of Applied Physiology and Occupational Physiology·J F GautierP J Lefèbvre
Feb 14, 2002·Journal of Applied Physiology·Stéphane CoutureFrançois Péronnet
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Dec 4, 2004·European Journal of Applied Physiology·Ronald RacetteCarole Lavoie
Sep 26, 2009·European Journal of Applied Physiology·Denis P BlondinFrançois Haman
Apr 25, 2012·Medicine and Science in Sports and Exercise·John P O'HaraRoderick F G J King
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Apr 12, 2001·Journal of Applied Physiology·Y BurelleF Péronnet
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Oct 13, 2020·Medicine and Science in Sports and Exercise·Alex GriffithsJohn P O'Hara
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Jul 27, 1999·Respiration Physiology·N PanteleevD Massicotte
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