Use of 5-cyano-2,3-ditolyl-tetrazolium chloride staining as an indicator of biocidal activity in a rapid assay for anti-Acanthamoeba agents.

Journal of Clinical Microbiology
Takeshi KobayashiYuichi Ohashi

Abstract

The usefulness of 5-cyano-2,3-ditolyl-tetrazolium chloride (CTC) staining to determine the respiratory activity of Acanthamoeba was evaluated in this study. Acanthamoeba trophozoites and cysts have a red fluorescence after staining with CTC. To determine the effectiveness of CTC staining as a CTC biocidal assay for Acanthamoeba, the trophozoites and cysts of Acanthamoeba castellanii (ATCC 5037) were treated with serial concentrations of disinfectant solutions, namely, polyhexamethylene biguanide (PHMB) and commercial soft contact lens (SCL) disinfectant solutions. The treated Acanthamoeba organisms were stained with CTC, and their respiratory activity was determined by the intensity of fluorescence in a fluorescence microplate reader. The survival rates of the same samples were determined by a culture-dependent biocidal assay using the Spearman-Karber method. Our results showed that the respiratory activities determined by the CTC biocidal assay and the survival rates determined by the culture-dependent biocidal assay for Acanthamoeba trophozoites and cysts decreased in a dose-dependent way after PHMB treatments, and the results were significantly correlated (r = 0.83 and P < 0.01 for trophozoites; r = 0.60 and P < 0.01 for cys...Continue Reading

References

Jun 1, 1992·Applied and Environmental Microbiology·G G RodriguezH F Ridgway
Mar 1, 1990·The British Journal of Ophthalmology·D F LarkinD L Easty
Jun 1, 1995·The British Journal of Ophthalmology·T B GrayP R Rose
Feb 1, 1995·Antimicrobial Agents and Chemotherapy·D PerrineM Robba
Aug 1, 1994·Applied and Environmental Microbiology·J J SmithG A McFeters
Aug 1, 1997·The Journal of Antimicrobial Chemotherapy·W KhunkittiA D Russell
Jun 23, 1998·Survey of Ophthalmology·C D Illingworth, S D Cook
Mar 4, 2000·Applied and Environmental Microbiology·R N BorazjaniD G Ahearn
Oct 29, 2002·Journal of Microbiological Methods·Véronique CréachBertrand Le Rouzic
Apr 15, 2003·Clinical Microbiology Reviews·Francine Marciano-Cabral, Guy Cabral
Dec 24, 2003·Investigative Ophthalmology & Visual Science·Simon KilvingtonMelville Matheson
Dec 8, 2005·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·R N Borazjani, S Kilvington
Aug 1, 1994·Applied and Environmental Microbiology·A WindingJ Sørensen
Jun 27, 2007·Cornea·Nuthida ThebpatiphatElisabeth J Cohen
Sep 18, 2007·Eye & Contact Lens·H Dwight Cavanagh
May 1, 2009·Journal of Clinical Microbiology·Stephanie P JohnstonGovinda Visvesvara
Dec 17, 2009·Eye & Contact Lens·James LonnenJacinto Santodomingo-Rubido
Jul 13, 2011·Japanese Journal of Ophthalmology·Takeshi KobayashiYuichi Ohashi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.