Use of 65 kDa mannoprotein gene primers in Real Time PCR identification of Candida albicans in biological samples

Molecular and Cellular Probes
Silvia AranciaFlavia De Bernardis

Abstract

A method for the detection and quantification of Candida albicans in biological samples (blood, urine and serum) was developed with the use of Real-Time PCR utilizing CaMP65-specific primers. Two different systems were used for the detection in the LightCycler platform (Roche): the SYBR green fluorescent dye with melting peak analysis and the 5'nuclease fluorescent-probe detection. The amplification was highly specific for C. albicans, providing no cross-reaction on genomic DNA extracted from other Candida species or Aspergillus. The sensitivity in simulated biological samples was especially high (1 genome) when applied to sera and urine, and in blood samples the limit of detection was higher by ten-fold. Finally, the real-time PCR was employed in order to detect and quantify C. albicans in the sera from patients with invasive candidiasis.

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Citations

May 26, 2009·Revista iberoamericana de micología·José Pontón
Apr 21, 2009·Clinics in Chest Medicine·Nancy L Wengenack, Matthew J Binnicker
May 19, 2007·Diagnostic Microbiology and Infectious Disease·Danièle MaubonChristine Aznar
Mar 17, 2007·Medical Mycology·D H PincusS Chatellier
Sep 25, 2009·Clinical and Vaccine Immunology : CVI·Rodrigo BerzaghiZoilo Pires de Camargo

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