PMID: 6164983Feb 25, 1981Paper

Use of a cloned double stranded cDNA coding for a major androgen dependent protein in rat seminal vesicle secretion: the effect of testosterone in gene expression

Nucleic Acids Research
P E ManssonS E Harris

Abstract

The abundant class of poly(A+)RNA [poly(A+)RNA11S] from rat seminal vesicle was used to synthesize ds-cDNA11S. The ds-cDNA11S was inserted and cloned into the Pst I site of pBR-322 using E. coli RR1 as host. Colony filter hybridization and restriction mapping was used to demonstrate that a 620 NTP long insert in a plasmid clone (pSV2) represents the almost full length structural gene coding for a precursor to the seminal vesicle secretion protein IV (SVS IV). The entire insert was sequenced and the coding region was matched with the known amino acid sequence. Most of the signal peptide sequence was derived from the DNA sequence. The insert in pSV2 was labelled and used to study the effect of testosterone on the accumulation of mRNA SVS IV. Administration of testosterone to castrated rats resulted in the induction of mRNA SVS IV from a few molecules per cell to levels of over 100,000 after 96 h of hormone treatment.

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Grunstein, D S Hogness

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Citations

Apr 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·Y TomookaJ A McLachlan
Jan 1, 1987·Journal of Steroid Biochemistry·J F CatterallB Funkenstein
Jun 1, 1982·Molecular and Cellular Endocrinology·S J HigginsJ Wilson
Aug 1, 1983·Molecular and Cellular Endocrinology·D B CarterS E Harris
Aug 1, 1984·Molecular and Cellular Endocrinology·S E Fawell, S J Higgins
Aug 1, 1986·Molecular and Cellular Endocrinology·J T MooreE D Wieben
Oct 1, 1986·Molecular and Cellular Endocrinology·J G DoddR J Matusik
Mar 1, 1987·Molecular and Cellular Endocrinology·S E FawellS J Higgins
Sep 1, 1988·Molecular and Cellular Endocrinology·A L SchifmanS E Harris
Sep 10, 1990·Molecular and Cellular Endocrinology·A MartinezC Jean
Apr 1, 1991·Molecular and Cellular Endocrinology·S J Higgins, A L Hemingway
Jan 14, 2004·European Journal of Biochemistry·Carlo CaporalePaola Stiuso
Dec 3, 1999·European Journal of Biochemistry·P StiusoR Ragone
Jul 4, 2001·European Journal of Biochemistry·V MetaforaS Metafora
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S E HarrisB Burkhart
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H Lilja, A Lundwall
May 25, 1983·Nucleic Acids Research·J C KandalaW S Kistler
Nov 18, 1997·European Journal of Biochemistry·A LundwallJ Malm
Jan 5, 2002·Clinical and Diagnostic Laboratory Immunology·Caterina Romano-CarratelliSalvatore Metafora
Dec 25, 2007·Journal of Translational Medicine·Biagio Di MiccoPierpaolo Di Micco
Sep 30, 2008·Pakistan Journal of Biological Sciences : PJBS·Amir Afshin KhakiAfshin Zahedi
Aug 1, 1995·The Journal of Experimental Zoology·R CarballadaP Esponda
Jun 1, 1989·International Journal of Andrology·T NormandC Jean
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Aug 3, 2013·Theriogenology·Naïma Kaci-OuchfounJean Marie Exbrayat
Sep 1, 1982·International Journal of Peptide and Protein Research·Y C Pan, S S Li

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