Use of a commercial protease and yeasts to obtain CGRP-like molecules from saithe protein

Journal of Agricultural and Food Chemistry
Oscar Martínez-AlvarezMartine Fouchereau-Peron

Abstract

Different bioactive molecules, such as CGRP-like peptides, can be found in fish protein hydrolysates. Calcitonin gene-related peptide (CGRP) is a neuropeptide known to act as a potent arterial and venous vasodilator in humans. This study focuses on the industrial obtaining of CGRP-like molecules from saithe (Pollachius virens) byproduct, derived from the filleting process. Protein from P. virens was primarily hydrolyzed with Alcalase and later treated with Saccharomyces cerevisiae live cells. Treatment with Saccharomyces doubled the quantity of bioactive molecules obtained. The CGRP-like molecules were partially purified by chromatography, and the immunoreactive material was further analyzed for its CGRP-like bioactivity, using a specific radioreceptor assay. The concentration of CGRP-like molecules increased over 100-fold after purification. The bioactive molecules were able to induce cyclic AMP stimulation in rat liver membranes. Finally, partial sequencing of the bioactive peptide was performed, showing some homology with alpha-actin and myosin of several fish species.

References

May 1, 1992·British Journal of Pharmacology·A AbdelrahmanC C Pang
Feb 4, 1990·Regulatory Peptides·M Fouchereau-PeronM S Moukhtar
Apr 15, 1988·Biochemical and Biophysical Research Communications·A YamaguchiT Fujita
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jun 1, 1995·Bioscience, Biotechnology, and Biochemistry·A OkamotoF Yanagida
Jun 13, 2002·Critical Reviews in Food Science and Nutrition·M GobbettiR Di Cagno
May 29, 2003·Current Pharmaceutical Design·H Korhonen, A Pihlanto
Dec 3, 2003·Advances in Food and Nutrition Research·Anne Pihlanto, Hannu Korhonen
Oct 20, 2005·Journal of Bioscience and Bioengineering·Toshiaki IchimuraSusumu Maruyama
Dec 27, 2005·European Journal of Pharmacology·Saurabh GuptaAntoinette Maassenvandenbrink
Aug 17, 2006·Journal of Physiology and Biochemistry·I Márquez-RodasG Balfagón
Jun 21, 2007·Journal of Agricultural and Food Chemistry·Oscar Martínez-AlvarezMartine Fouchereau-Peron

❮ Previous
Next ❯

Citations

Nov 23, 2015·Applied Biochemistry and Biotechnology·I GodinhoI Batista
Jun 26, 2020·World Journal of Microbiology & Biotechnology·Gabriela Vollet MarsonMiriam Dupas Hubinger
Jan 20, 2015·Journal of Agricultural and Food Chemistry·Leticia Mora, Maria Hayes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Agricultural and Food Chemistry
Oscar Martínez-AlvarezMartine Fouchereau-Peron
Trends in Pharmacological Sciences
Henri DoodsStefan Just
CNS & Neurological Disorders Drug Targets
Lars Edvinsson, Kenneth Ahrend Petersen
© 2022 Meta ULC. All rights reserved