Use of a dialyzable short-chain phospholipid for efficient solubilization and reconstitution of influenza virus envelopes

Biochimica Et Biophysica Acta
Jørgen de JongeAnke Huckriede

Abstract

Virosomes are reconstituted viral envelopes that can serve as vaccines and as vehicles for cellular delivery of various macromolecules. To further advance the use of virosomes, we developed a novel dialysis procedure for the reconstitution of influenza virus membranes that is easily applicable to industrial production and compatible with encapsulation of a variety of compounds. This procedure relies on the use of 1,2-dicaproyl-sn-glycero-3-phosphocholine (DCPC) as a solubilizing agent. DCPC is a short-chain lecithin with detergent-like properties and with a critical micelle concentration of 14 mM. DCPC effectively dissolved the influenza virus membranes after which the nucleocapsids could be removed by ultracentrifugation. The solubilized membrane components were reconstituted either by removal of DCPC by dialysis or by a procedure involving initial dilution of the solubilized membrane components followed by dialysis. Both protocols resulted in removal of 99.9% of DCPC and simultaneous formation of virosomes. Analysis of the virosome preparations by equilibrium sucrose density gradient centrifugation revealed co-migration of phospholipid and protein for virosomes produced by either method. Moreover, both virosome preparations s...Continue Reading

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Citations

Mar 14, 2008·Pharmaceutical Research·J-P AmorijW L J Hinrichs
Sep 29, 2009·Biophysical Chemistry·Daniel J LairdJennifer A Whiles Lillig
Jul 14, 2007·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Jørgen de JongeHenderik W Frijlink
Nov 21, 2007·Journal of Liposome Research·Jan WilschutHenderik W Frijlink
Apr 25, 2007·Journal of Liposome Research·Anke HuckriedeJan Wilschut
Mar 16, 2007·The Biochemical Journal·Jørgen de JongeAnke Huckriede
Jan 1, 2013·Molecular and Cellular Therapies·Babak JalilianThomas Vorup-Jensen
Mar 16, 2021·Journal of Microencapsulation·Varun KumarSuman Nagpal

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