Use of a feline respiratory epithelial cell culture system grown at the air-liquid interface to characterize the innate immune response following feline herpesvirus 1 infection

Virus Research
Rahul K NelliGisela Soboll Hussey

Abstract

Infection with feline herpesvirus-1 (FHV-1) accounts for 50% of viral upper respiratory diseases in domestic cats and is a significant cause of ocular diseases. Despite the clinical significance and high prevalence of FHV-1 infection, currently available vaccines cannot completely protect cats from infection and lifelong latency. FHV-1 infects via the mucous membranes and replicates in respiratory epithelial cells, but very little is known about the early innate immunity at this site. To address questions about immunity to FHV-1, feline respiratory epithelial cells cultured at air-liquid interface (ALI-FRECs) were established by collecting respiratory tracts from 6 healthy cats after euthanasia. Cells were isolated, cultured and characterized histologically and immunologically before infection with FHV-1. The expression of Toll-like receptors (TLRs), cytokine and chemokine responses were measured by real time PCR. ALI-FRECs morphologically resembled the natural airways of cats with multilayered columnar epithelial cells and cilia. Immunological properties of the natural airways were maintained in ALI-FRECs, as evidenced by the expression of TLRs, cytokines, chemokines, interferons, beta-defensins, and other regulatory genes. Fu...Continue Reading

References

Jan 26, 1991·The Veterinary Record·D A HarbourR M Gaskell
Apr 1, 1984·Environmental Health Perspectives·R Breeze, M Turk
Oct 6, 1998·Annual Review of Biochemistry·G R StarkR D Schreiber
Nov 24, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A WeinbergB A Dale
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 19, 2003·Journal of Feline Medicine and Surgery·J E FoleyC Leutenegger
Jul 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hubertus HochreinHermann Wagner
Jun 21, 2005·Veterinary Immunology and Immunopathology·Glicerio IgnacioGregg A Dean
May 4, 2006·Fiziologiia cheloveka·K A Lebedev, I D Poniakina
Oct 14, 2006·Uirusu·Satoshi Uematsu, Shizuo Akira
Nov 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ayuko SatoAkiko Iwasaki
Aug 19, 2007·The Journal of Infectious Diseases·Mirella SalvatoreMary E Klotman
Sep 18, 2007·Science·Shen-Ying ZhangJean-Laurent Casanova
Dec 20, 2007·The Journal of General Virology·Richard E Randall, Stephen Goodbourn
Jun 2, 2009·Journal of Feline Medicine and Surgery·Etienne ThiryMarian C Horzinek
Feb 20, 2010·Interdisciplinary Perspectives on Infectious Diseases·Guey-Chuen Perng, Clinton Jones
Feb 5, 2011·Veterinary Immunology and Immunopathology·Ayshea M QuintanaGisela Soboll Hussey
Apr 26, 2011·Journal of Feline Medicine and Surgery·David Gould
Feb 14, 2012·Current Opinion in Virology·John W Schoggins, Charles M Rice
Feb 22, 2012·Nature Immunology·Nan Yan, Zhijian J Chen
Apr 26, 2012·Influenza and Other Respiratory Viruses·Allen C BatemanChristopher W Olsen
Jan 5, 2013·Veterinary Immunology and Immunopathology·Sanne Maria Johanna DolieslagerMarcello Pasquale Riggio
Oct 8, 2013·Journal of Molecular Biology·Sarah S WilsonJason G Smith
Feb 25, 2014·Veterinary Microbiology·Gisela Soboll HusseyGabriele Landolt
Jul 18, 2014·PLoS Pathogens·Mayim E WiensJason G Smith
Sep 23, 2014·Nature Reviews. Immunology·Michael J HoltzmanXinyu Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antigenic Modulation

Antigenic modulation occurs when an antibody cross-links antigens on a cell surface, causing the antigens to become internalized. This can lead to therapeutic failure of monoclonal antibodies as the expression of the antigen becomes decreased on target cells. Find the latest research on antigenic modulation here.

Acute viral rhinopharyngitis

Acute viral rhinopharyngitis, also known as "common cold", is an acute, self-limiting viral infection of the upper respiratory tract involving the nose, sinuses, pharynx and larynx. Discover the latest research on acute viral rhinopharyngitis here.