Use of a flow cell bioreactor as a chronic toxicity model system

Toxicology in Vitro : an International Journal Published in Association with BIBRA
A B HanleyE Opara

Abstract

We describe the use of a model system to mimic chronic toxin exposure, similar to that which might be found in a human situation, where exposure to dietary or environmental toxins occurs at a low level for an extended period of time. This is in contrast to the acute, immediately toxic dose effect usually observed in flask tissue culture. The apparatus used was a flow cell bioreactor in which cells can be cultured for lengthy periods of time as a continuous viable population. The compound used as the toxic agent was 3-nitropropanoic acid (3-NPA), a fungal neurotoxin which acts as a suicide inhibitor of succinate dehydrogenase. The secondary human cell line, Int 407, was tested in conventional flask culture for periods of up to 72 hours to determine the no-observed-effect level (NOEL) for 3-NPA. Cell populations established in the bioreactor were continuously exposed to levels of 3-NPA below that of the NOEL for periods of up to 4 weeks and the viability of the population determined using MTT, trypan blue and ATP assays.

References

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Citations

Aug 17, 2001·Alternatives to Laboratory Animals : ATLA·C L Broadhead, R D Combes
Jan 7, 2003·Alternatives to Laboratory Animals : ATLA·Pilar Prieto
Jul 13, 2006·Alternatives to Laboratory Animals : ATLA·Pilar PrietoWalter Pfaller
Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·Pilar PrietoCarl Westmoreland
Jul 6, 2019·Frontiers in Bioengineering and Biotechnology·Joana Costa, Arti Ahluwalia
Dec 17, 2008·Alternatives to Laboratory Animals : ATLA·Christina GrindonJohn F Garrod
Jul 12, 2013·International Journal of Cosmetic Science·I GarciaN Domloge
Mar 13, 2008·Alternatives to Laboratory Animals : ATLA·Christina GrindonJohn F Garrod
Nov 10, 2016·Sensors·Guojun ZhangWendong Zhang
Jun 9, 2004·International Journal of Occupational Safety and Ergonomics : JOSE·Jolanta Skowroń, Lidia Zapór
Mar 15, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G EisenbrandJ Kleiner

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