Use of a glycerol-limited, long-term chemostat for isolation of Escherichia coli mutants with improved physiological properties.

Microbiology
C WeikertJames E Bailey

Abstract

The evolution of Escherichia coli MG1655 mutants was followed over 126 d in a glycerol-limited chemostat at a dilution rate of 0.05 h-1. This corresponds to a total of 217 generations at a doubling time of 13.9 h. After this time, nearly 90% of the chemostat population consisted of evolved mutant strains as determined by their altered colony morphologies on plates. Two mutants were isolated that exhibited generally improved growth phenotypes in batch cultivations on glycerol, glucose or the gluconeogenic substrate acetate. Higher specific growth rates and increased biomass yields were found for both mutants. For one mutant, this behaviour was combined with significantly reduced secretion of overflow metabolites when either glycerol or glucose was the carbon source. Additionally, during all growth phases of a batch cultivation, this mutant exhibited increased resistance to a variety of adverse conditions including heat shock, osmotic stress and nutrient deprivation. It also displayed significantly shorter lag phases.

References

Jan 1, 1992·Society for Applied Bacteriology Symposium Series·A Matin
Jan 1, 1992·Annual Review of Genetics·C G Kurland
Jan 1, 1989·Annual Review of Microbiology·A MatinJ E Schultz
Jun 1, 1983·Microbiological Reviews·D E Dykhuizen, D L Hartl
Dec 1, 1950·Proceedings of the National Academy of Sciences of the United States of America·A NOVICK, L SZILARD
Jun 5, 1994·Biotechnology and Bioengineering·N N DedhiaJ E Bailey
May 1, 1981·Evolution; International Journal of Organic Evolution·Daniel Dykhuizen, Daniel Hartl

❮ Previous
Next ❯

Citations

Apr 1, 1999·Biotechnology and Bioengineering·C WeikertJ E Bailey
Dec 18, 2001·Biotechnology and Bioengineering·Jeremy S EdwardsBernhard O Palsson
Jun 16, 2007·Applied Microbiology and Biotechnology·C Perry Chou
Aug 3, 2011·Applied Microbiology and Biotechnology·Thomas MaskowJohannes Lerchner
Feb 1, 2011·Antonie van Leeuwenhoek·Mustafa SenZ Petek Cakar
May 19, 2005·Biotechnology Advances·Joseph Shiloach, Rephael Fass
Oct 29, 2003·Current Opinion in Biotechnology·Ryan T Gill
Feb 2, 2002·Applied and Environmental Microbiology·Joseph J ArensdorfPhilip T Pienkos
Apr 5, 2003·Applied and Environmental Microbiology·Marco Sonderegger, Uwe Sauer
Jul 3, 2013·Microbial Cell Factories·Martin Dragosits, Diethard Mattanovich
May 8, 2014·Environmental Science & Technology·Allison M SpeersGemma Reguera
Mar 23, 2005·FEMS Yeast Research·Z Petek CakarUwe Sauer
Jun 9, 2009·Biotechnology Advances·Arnold L Demain, Preeti Vaishnav
Aug 12, 2003·Biotechnology and Bioengineering·Peter Steiner, Uwe Sauer
Apr 11, 2009·Biotechnology Journal·Zeynep Petek Cakar
Aug 24, 2007·Journal of Evolutionary Biology·I GudeljR C MacLean
Oct 7, 2006·Biotechnology Progress·K ListnerM Chartrain
Feb 22, 2005·Metabolic Engineering·Marco SondereggerUwe Sauer
Jun 23, 2004·Research in Microbiology·Julian Adams
Apr 20, 2006·Trends in Microbiology·Engràcia CostaJan-Ulrich Kreft
May 18, 2010·Journal of Theoretical Biology·Dirk KoschützkiFalk Schreiber
May 21, 1999·The Journal of Biological Chemistry·J A DiderichA L Kruckeberg
Sep 10, 2020·Frontiers in Bioengineering and Biotechnology·Stefan KittlerChristoph Slouka
Apr 7, 2018·Applied Microbiology and Biotechnology·Zhengming ZhuGuocheng Du
Jun 22, 2017·FEMS Microbiology Reviews·Herwig BachmannBas Teusink
Sep 11, 1999·Journal of Bacteriology·E R Zinser, R Kolter

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Applied Microbiology and Biotechnology
J Nielsen
Applied and Environmental Microbiology
O SelifonovaV Schellenberger
© 2021 Meta ULC. All rights reserved