Use of a human plaque-forming cell assay to study peripheral blood bursa-equivalent cell activation and excessive suppressor cell activity in humoral immunodeficiency

The Journal of Clinical Investigation
H G Herrod, R H Buckley

Abstract

A plaque assay that detects human mononuclear blood cells producing immunoglobulin (Ig)M antibody to sheep erythrocytes was investigated for its usefulness in studying B-cell activation and regulation in 24 patients with humoral immunodeficiency. Cells from 3 of 15 patients with common variable agammaglobulinemia produced some plaques (range 40--160/10(6) cells; normal range 80--1240/10(6)), but those from the other 12, from all 7 with x-linked agammaglobulinemia and from the 2 with x-linked immunodeficiency with hyper-IgM failed to produce any detectable plaques. In co-cultures of patient and normal cells a very good correlation was seen between results of the plaque assay and an IgM biosynthesis assay in detecting excessive suppressor cell activity. Cells from 7 of 15 common variable agammaglobulinemics, from 3 of 7 x-linked agammaglobulinemics, and from both patients with hyper-IgM caused significant suppression of IgM biosynthesis and(or) plaque formation by normal cells. The observations in the last two groups and discordance for excess suppressor activity in identical twins with common variable agammaglobulinemia suggest that the activity develops secondarily to whatever their primary defects may be. Culturing non-T cells...Continue Reading

References

Jan 1, 1976·Journal of Immunological Methods·H M Dosch, E W Gelfand
Jun 30, 1977·The New England Journal of Medicine·C M WilfertF S Rosen
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A S Fauci, K R Pratt
Jul 1, 1976·The Journal of Clinical Investigation·F P SiegalR A Good
Sep 1, 1976·The Journal of Experimental Medicine·A L LuzzatiB Pernis
May 3, 1973·The New England Journal of Medicine·M D CooperR J Wedgwood
Dec 1, 1973·The Journal of Clinical Investigation·L Y WuM D Cooper
Jan 1, 1974·Transplantation Reviews·N K JerneI Lefkovits

❮ Previous
Next ❯

Citations

Oct 1, 1982·Journal of Clinical Immunology·H G Herrod, W R Valenski
Feb 18, 1982·The New England Journal of Medicine·H M DoschE W Gelfand
Jan 1, 1985·The Journal of Experimental Medicine·L MayerH G Kunkel
Nov 1, 1983·The Journal of Clinical Investigation·D LevittM D Cooper
Dec 1, 1983·The Journal of Clinical Investigation·E van LoghemL Kater
Aug 1, 1991·Clinical Immunology and Immunopathology·H G HerrodM E Dockter
Feb 1, 1983·Immunology Today·A H Filipovich, J H Kersey
Nov 1, 1994·Metabolism: Clinical and Experimental·C K Buffington, A E Kitabchi
Sep 1, 1990·Scandinavian Journal of Immunology·L ThorsteinssonO Jensson
Feb 1, 1983·Scandinavian Journal of Haematology·P Tauris, P W Hansen
Nov 1, 1983·JPEN. Journal of Parenteral and Enteral Nutrition·R A HelmsM L Christensen
May 1, 1994·Metabolism: Clinical and Experimental·C K BuffingtonA E Kitabchi

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cell Activation

B cell activation is initiated by the ligation of the B cell receptor with antigen and ultimately results in the production of protective antibodies against potentially pathogenic invaders. Here is the latest research.