Use of a lux-based procedure to rapidly visualize root colonisation by Pseudomonas fluorescens in the wheat rhizosphere

Antonie van Leeuwenhoek
L A de WegerBen Lugtenberg

Abstract

The bioluminescently marked Pseudomonas fluorescens strain 5RL, has been used previously to follow colonisation of soy bean roots (De Weger et al. [1991] Appl. Environ. Microbiol. 57:36-41). In the present paper the method has been further developed and optimized for wheat roots and it is used to get a quick overview of the colonisation patterns of many different root systems at the same time. Colonisation was followed on wheat plants grown in our gnotobiotic sand system (Simons et al., 1996. Mol Plant Microbe Interact 9: 600-607) and the following results were obtained. (i) A spatio-temporal analysis of the colonisation of wheat roots showed that 4 days after planting the highest bacterial activity was observed at the upper part of the root. After 6 days the high bacterial activity at the upper part was further increased, whereas spot-like activities were observed on the lower root parts, possibly due to micro-colonies. (ii) Bacterial mutations causing lack of motility or auxotrophy for amino acids resulted in impaired colonisation of the lower root parts, indicating that motility and prototrophy for the involved amino acid(s) are important factors for wheat root colonisation by strain 5RL. (iii) Coinoculation of strain 5RL wi...Continue Reading

Citations

Nov 10, 2001·Annual Review of Phytopathology·B J LugtenbergG V Bloemberg
Nov 9, 2002·Molecular Plant-microbe Interactions : MPMI·Sandra de WeertBen J J Lugtenberg
Nov 5, 2005·Applied and Environmental Microbiology·Pascal MirleauMichael A Kertesz
Dec 23, 2011·Canadian Journal of Microbiology·Caressa J CaldwellDarren R Korber

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