PMID: 1198406Nov 15, 1975Paper

Use of a lyophilized reference plasma to compare coagulation test procedures: Normotest, Simplastin-A and Thrombotest

Thrombosis Et Diathesis Haemorrhagica
J Kahan, I Norén

Abstract

In 4 collaborative trials, involving a varying number of hospital laboratories in the Stockholm area, the coagulation activity of different test materials was estimated with the one-stage prothrombin tests routinely used in the laboratories, viz. Normotest, Simplastin-A and Thrombotest. The test materials included different batches of a lyophilized reference plasma, deep-frozen specimens of diluted and undiluted normal plasmas, and fresh and deep-frozen specimens from patients on long-term oral anticoagulant therapy. Although a close relationship was found between different methods, Simplastin-A gave consistently lower values than Normotest, the difference being proportional to the estimated activity. The discrepancy was of about the same magnitude on all the test materials, and was probably due to a divergence between the manufacturers' procedures used to set "normal percentage activity", as well as to a varying ratio of measured activity to plasma concentration. The extent of discrepancy may vary with the batch-to-batch variation of thromboplastin reagents. The close agreement between results obtained on different test materials suggests that the investigated reference plasma could be used to calibrate the examined thrombopla...Continue Reading

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