PMID: 2499618Jul 1, 1989Paper

Use of a monoclonal rat anti-mouse Ig light chain (RAMOL-1) antibody reduces background binding in immunohistochemical and fluorescent antibody analysis

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
N T BrodinH O Sjögren

Abstract

Rat monoclonal antibodies (MAb) directed to mouse Ig heavy and light chain determinants were produced. A rat anti-mouse light chain MAb (RAMOL-1) which bound to all (24/24) mouse Ig of the kappa light chain type and with varying strength to 4/4 lambda light chain-bearing Ig was evaluated as a general secondary reagent, together with two MAb that bound to the heavy chain of mouse IgG. They were conjugated with biotin or FITC and used in immunohistochemical and immunofluorescence assays to detect mouse monoclonal antibodies binding to antigens expressed in rat and human tissues and cells. As compared to commercially available polyclonal reagents, RAMOL-1 gave higher staining contrast by showing lower background staining and equal or higher staining of the primary MAb tested. This was a result of two main effects. First, crossreactivity with endogenous Ig and tissue type-specific determinants was eliminated. With polyclonal anti-mouse Ig reagents, binding to endogenous Ig was noted in vascular spaces and on Ig-bearing cells, and to rat gastric mucosa and epithelial tumor tissue in frozen tissue sections, even when diluted in high concentrations of serum homologous to the tissue. Second, binding of the secondary reagent was reduced...Continue Reading

Citations

Nov 13, 2003·Experimental and Molecular Pathology·Lars Bryngelson OhlssonMagnus Lindvall
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·M DohlstenP Lind
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·M DohlstenT Kalland
Sep 1, 1990·Scandinavian Journal of Immunology·K KristenssonR Carlsson
Sep 5, 2007·Cancer Letters·Hua LiuBengt Widegren
Feb 15, 1989·International Journal of Cancer. Journal International Du Cancer·N T BrodinH O Sjögren
Aug 30, 2012·The Journal of Trauma and Acute Care Surgery·Philip F Stahel
Apr 7, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Erika ElgströmSophie E Eriksson
May 15, 1990·International Journal of Cancer. Journal International Du Cancer·N T BrodinH O Sjögren
Aug 11, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wiaam BadnHans Olov Sjögren

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