Use of a new strategy to isolate and characterize 436 Drosophila cDNA clones corresponding to RNAs detected in adult heads but not in early embryos

Neuron
M J PalazzoloE M Meyerowitz

Abstract

We describe a new strategy for producing tissue-specific cDNA libraries and subsequently identifying tissue-specific clones. This method was used to screen for cDNA clones corresponding to RNAs expressed in the Drosophila head that cannot be detected in the early embryo. RNA blots were used to assess the spatial and temporal patterns of expression of these RNAs. The ensemble of 436 head-not-embryo clones identified roughly 700 distinct RNAs that are differentially expressed in the Drosophila head. The RNA expression patterns can be classified into five major categories. it is argued that this ensemble of clones represents a large fraction of all genes differentially expressed in the adult head, but not detected in the early embryo. Many of these genes are likely to encode eye- and nervous system-specific products.

References

Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G H Travis, J G Sutcliffe
Apr 1, 1985·Cell·J E O'TousaM L Applebury
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S C FujitaS L Shotwell

❮ Previous
Next ❯

Citations

Jan 1, 1993·Archives of Insect Biochemistry and Physiology·D Segal
Jun 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K Kaiser
Feb 1, 1994·Journal of Neurobiology·D Raha, J Carlson
Nov 1, 1991·Trends in Neurosciences·R RanganathanC S Zuker
Sep 1, 1992·Trends in Neurosciences·K Kato
Feb 1, 1992·Trends in Genetics : TIG·D L HartlI W Duncan
Sep 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D L Hartl, E R Lozovskaya
Aug 1, 1993·Zygote : the Biology of Gametes and Early Embryos·D Ding, H D Lipshitz
Mar 1, 1993·Nature Genetics·J M Sikela, C Auffray
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D G Ballinger, S Benzer
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D R HydeS Benzer
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R N Van GelderJ H Eberwine
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K Kaiser, S F Goodwin
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M J PalazzoloD L Hartl
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B A HamiltonE M Meyerowitz
Apr 25, 1991·Nucleic Acids Research·B A HamiltonE M Meyerowitz
Apr 24, 1999·Genes & Development·X DongS L Zipursky
Mar 1, 1993·Postgraduate Medical Journal·L M Drummond
Jun 23, 1995·The Journal of Biological Chemistry·S Shah, D R Hyde
Feb 8, 2007·Molecular Genetics and Genomics : MGG·Kirk L Mecklenburg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
D G Ballinger, S Benzer
Proceedings of the National Academy of Sciences of the United States of America
D R HydeS Benzer
© 2022 Meta ULC. All rights reserved