Use of a novel real-time PCR technique to monitor and quantitate Mycoplasma bovis infection in cattle herds with mastitis and respiratory disease

The Veterinary Journal
K SachseHelmut Hotzel

Abstract

Mycoplasma bovis infection of cattle tends to persist in affected herds and can be resistant to treatment. Given that the level of shedding of the organism can reflect the state of ongoing infection, a study was established to quantify the degree of such shedding in milk samples from infected herds using a novel real-time PCR technique. While the M. bovis load in herds with clinical disease was significantly higher than in disease-free herds (P<0.001), infection persisted in the latter. Similarly, M. bovis was detected more frequently in conjunctival swabs from calves with respiratory disease when compared with samples from animals that did not exhibit such clinical signs. This novel real-time PCR assay is specific for M. bovis and can detect bacterial loads as low as 100 colony forming units/mL of milk.

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Citations

Oct 5, 2011·Journal of Mammary Gland Biology and Neoplasia·Ruth N ZadoksYnte H Schukken
Oct 30, 2012·The Veterinary Clinics of North America. Food Animal Practice·Abhijit GurjarPaolo Moroni
May 9, 2012·Veterinary Microbiology·Ashley G BoyleShelley C Rankin
Mar 13, 2010·The Veterinary Journal·Hywel J Ball, Robin A J Nicholas
Sep 26, 2012·Australian Veterinary Journal·P M HickP D Kirkland
Jul 13, 2011·Journal of Veterinary Internal Medicine·F P MaunsellE D Janzen
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Sep 3, 2013·Veterinary Microbiology·Lucas M MarquesJorge Timenetsky
Oct 1, 2016·The Veterinary Journal·Robin A J NicholasInna Lysnyansky
Jul 12, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Colin J BellHywel J Ball
Jan 12, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Gerard M MurrayJoseph P Cassidy
Jan 11, 2017·The Journal of Veterinary Medical Science·Mai KishimotoTetsuya Mizutani
Oct 10, 2015·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Harald PothmannMarc Drillich
Jan 18, 2018·Tropical Animal Health and Production·S BeheraD Jena
Sep 22, 2018·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·John D LoyBing Wang
Sep 1, 2018·BMC Veterinary Research·Mette Bisgaard PetersenLiza Rosenbaum Nielsen
Apr 20, 2018·Journal of Veterinary Internal Medicine·Alysia M ParkerJohn K House
Apr 30, 2011·The Veterinary Record·R A J Nicholas
Dec 15, 2020·Journal of Dairy Science·Vera HaapalaHeli Simojoki
Jul 13, 2021·Frontiers in Veterinary Science·Nicole B GoeckeLars E Larsen
Jan 14, 2022·Veterinary Research·Jianmin ChaiJiangchao Zhao

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