Use of a pooled transposon mutation grid to demonstrate roles in disease development for Erwinia carotovora subsp. atroseptica putative type III secreted effector (DspE/A) and helper (HrpN) proteins

Molecular Plant-microbe Interactions : MPMI
M C HolevaIan Toth

Abstract

Soft rot Erwinia spp., like other closely related plant pathogens, possess a type III secretion system (TTSS) (encoded by the hrp gene cluster) implicated in disease development. We report the sequence of the entire hrp gene cluster and adjacent dsp genes in Erwinia carotovora subsp. atroseptica SCRI1039. The cluster is similar in content and structural organization to that in E. amylovora. However, eight putative genes of unknown function located within the E. carotovora subsp. atroseptica cluster do not have homologues in the E. amylovora cluster. An arrayed set of Tn5 insertional mutants (mutation grid) was constructed and pooled to allow rapid isolation of mutants for any given gene by polymerase chain reaction screening. This novel approach was used to obtain mutations in two structural genes (hrcC and hrcV), the effector gene dspE/A, and the helper gene hrpN. An improved pathogenicity assay revealed that these mutations led to significantly reduced virulence, showing that both the putative E. carotovora subsp. atroseptica TTSS-delivered effector and helper proteins are required for potato infection.

References

May 1, 1996·Molecular Microbiology·A J BogdanoveF Van Gijsegem
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·A J BogdanoveS V Beer
Jul 8, 1998·FEBS Letters·S GaudriaultM A Barny
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·B J AkerleyJ J Mekalanos
Dec 20, 2000·Bioinformatics·K RutherfordB Barrell
Dec 21, 2000·Trends in Microbiology·N Judson, J J Mekalanos
Mar 30, 2001·Molecular Plant-microbe Interactions : MPMI·H MorI Barash
Apr 3, 2001·Genome Research·D GordonP Green
May 31, 2002·Molecular Plant-microbe Interactions : MPMI·Ching-Hong YangNoel T Keen
Nov 18, 2003·Annual Review of Genetics·Finbarr Hayes
Nov 18, 2003·Molecular Microbiology·Roger Innes
Nov 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michael A JacobsColin Manoil
Jan 1, 2003·Molecular Plant Pathology·Ian K TothPaul R J Birch

❮ Previous
Next ❯

Citations

Jun 23, 2005·Current Opinion in Plant Biology·Ian K Toth, Paul R J Birch
Dec 30, 2011·Molecular Plant-microbe Interactions : MPMI·Isabel Rio-AlvarezEmilia López-Solanilla
Nov 2, 2012·Molecular Plant-microbe Interactions : MPMI·Marion F CubittGeorge P C Salmond
Nov 7, 2008·Molecular Plant-microbe Interactions : MPMI·J D GlasnerA O Charkowski
Jul 12, 2008·Molecular Plant-microbe Interactions : MPMI·A DegraveM-A Barny
May 19, 2009·Molecular Plant-microbe Interactions : MPMI·Jong Hyun HamDavid L Coplin
Mar 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Anne M L BarnardGeorge P C Salmond
May 5, 2009·Applied and Environmental Microbiology·Hye-Sook KimAmy O Charkowski
May 18, 2006·Annual Review of Phytopathology·Ian K TothPaul R J Birch
Jun 19, 2012·Annual Review of Phytopathology·Amy CharkowskiIris Yedidia
Aug 7, 2010·Annual Review of Phytopathology·David J Schneider, Alan Collmer
May 1, 2008·Plant Signaling & Behavior·David Reboutier, François Bouteau
Feb 21, 2013·FEMS Microbiology Letters·Sabrina SiamerMarie-Anne Barny
Jan 13, 2010·Molecular Plant-microbe Interactions : MPMI·Meriam TertaFrançois Bouteau

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.