Use of a quartz crystal microbalance to monitor immunoliposome--antigen interaction

Analytical Chemistry
K YunM Aizawa

Abstract

We have used quartz crystal microbalance (QCM)-based real-time biospecific interaction measurement to analyze the binding of immunoliposomes to antigen and examined the use of liposomes as signal-enhancing reagents in competitive QCM immunoassay. For the preparation of immunoliposomes, various amounts of bacterially produced lipid-tagged single-chain antibody against 2-phenyloxazolone were incorporated in phosphatidylcholine liposomes. The immunoliposomes bound specifically to immobilized hapten, and this binding was inhibited by soluble hapten in a concentration-dependent manner. In this competitive assay, antigen could be measured in the concentration range from 10(-5) to 10(-8) M.

Citations

Jul 16, 2010·Applied Biochemistry and Biotechnology·Eiry KobatakeMasayasu Mie
May 1, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Takeshi TeradaMitsuru Hashida
May 15, 2004·Biosensors & Bioelectronics·Thong-Yueh LinTse-Chuan Chou
Sep 6, 2006·Journal of Bioscience and Bioengineering·HoSup JungTomoji Kawai
Aug 16, 2012·Journal of Laboratory Automation·Kin Fong Lei

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