Use of a rat ex-vivo testis culture method to assess toxicity of select known male reproductive toxicants

Reproductive Toxicology
Keith M GoldsteinThomas K Baker

Abstract

Due to the complex physiology of the testes, in vitro models have been largely unsuccessful at modeling testicular toxicity in vivo. We conducted a pilot study to evaluate the utility of the Durand ex vivo rat seminiferous tubule culture model [1-3] that supports spermatogenesis through meiosis II, including the formation of round spermatids. We used this system to evaluate the toxicity of four known testicular toxicants: 1,3-dinitrobenzene (DNB), 2-methoxyacetic acid (MAA), bisphenol A (BPA), and lindane over 21 days of culture. This organotypic culture system demonstrated the ability to successfully model in vivo testicular toxicity (Sertoli cell toxicity and disruption of meiosis) for all four compounds. These findings support the application of this system to study molecules and evaluate mechanisms of testicular toxicity.

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Citations

Jun 14, 2017·Journal of Applied Toxicology : JAT·Agnieszka Tomza-MarciniakBogumiła Pilarczyk
Jun 26, 2020·Frontiers in Endocrinology·Luca De ToniCarlo Foresta
Dec 28, 2017·Human Reproduction Update·João Pedro Alves-Lopes, Jan-Bernd Stukenborg
Jan 27, 2021·Environmental Science and Pollution Research International·Antonine BlondetPhilippe Durand
May 1, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Andreas NatschHeike Laue
Jun 4, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Philippe DurandMarie-Hélène Perrard

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