Use of a serum-free reconstituted epidermis as a skin pharmacological model

Toxicology in Vitro : an International Journal Published in Association with BIBRA
O DoucetL Zastrow

Abstract

This study is aimed at assessing some of the possibilities offered by a new kind of three-dimensional skin culture in the field of toxicity and skin inflammation. The Reconstituted Epidermis used is presently the only skin model on the market in which normal human keratinocytes are allowed to grow and to differentiate in a chemically defined culture medium. The response of this original tissue to chemical (anionic and cationic surfactants) and physical (UVB radiation) insults was compared with human keratinocytes in monolayer culture and assessed through histological analysis, cell viability quantification and interleukin-1alpha (IL-1alpha) release measurement. For the surfactants and the UVB radiation a clear relationship was shown between the amount of IL-1alpha released and both the cell viability and the histological appearance of the skin. Sublethal doses of UV radiation induced exclusively an increase of intracellular amount of IL-1alpha, the magnitude of which seemed to be time dependent. The irritating potential of various kinds of cosmetic creams was then assessed using this skin model and compared with human irritation data. The intensity of erythema and oedema induced by a 48-hr human patch test was consistent with t...Continue Reading

References

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Citations

Oct 22, 2005·Toxicology and Applied Pharmacology·Sari PappinenArto Urtti
Mar 4, 2000·International Journal of Pharmaceutics·C S MaiaM Schäfer-Korting
Aug 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·O DoucetL Zastrow
Dec 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A CoquetteY Poumay
May 20, 2014·PloS One·Cameron K PostnikoffMaud B Gorbet
Apr 3, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sari PappinenArto Urtti
Jan 19, 2010·International Journal of Oral and Maxillofacial Surgery·T TobitaS E Feinberg
Sep 8, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Carine TornierNathalie Alépée
Sep 8, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nathalie AlépéeAnne de Brugerolle de Fraissinette
Oct 11, 2003·Photodermatology, Photoimmunology & Photomedicine·Christelle GélisPatricia Vicendo
Oct 26, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·O DoucetL Zastrow
Jul 18, 2008·Experimental Dermatology·György ParaghGerd Schmitz
Oct 19, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Carine TornierHoward I Maibach

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