PMID: 6409670Aug 8, 1983Paper

Use of a trapped fluorescent indicator to demonstrate effects of thyroliberin and dopamine on cytoplasmic calcium concentrations in bovine anterior pituitary cells

FEBS Letters
J G Schofield

Abstract

The fluorescent calcium indicator 'quin2' was used to demonstrate changes in cytoplasmic calcium concentrations in bovine anterior pituitary cells. The basal calcium concentration was 0.21 +/- 0.02 microM (mean of 4 cell preparations). Thyroliberin (TRH) (10(-10) - 10(-6) M) rapidly and at the higher concentrations transiently increased the concentration. Dopamine (10(-10) - 10(-7) M) decreased the concentration transiently and more slowly. At 10(-5) M, dopamine prevented the increase in calcium concentration caused by 10(-9) M TRH, and partially inhibited the increase caused by higher concentrations of the peptide. The data support the hypothesis that calcium is the second messenger for TRH, and suggest that dopamine inhibits TRH-induced prolactin secretion by preventing the calcium concentration from exceeding the level necessary to increase secretion.

References

Oct 1, 1981·Physiological Reviews·A Tixier-Vidal, D Gourdji
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P OnaliE Costa
Nov 1, 1982·Molecular and Cellular Endocrinology·K P Ray, M Wallis

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Citations

Jan 1, 1992·Journal of Neural Transmission. General Section·P Onali, M C Olianas
Nov 1, 1988·The Journal of Membrane Biology·S MuallemT G Beeker
Jan 1, 1994·Pharmacology & Therapeutics·D M Jackson, A Westlind-Danielsson
Feb 1, 1989·Trends in Pharmacological Sciences·L Vallar, J Meldolesi
Jan 1, 1989·Progress in Neurobiology·N Ben-JonathanJ F Hyde
Jun 1, 1984·Molecular and Cellular Endocrinology·K P Ray, M Wallis
Mar 1, 1984·Molecular and Cellular Endocrinology·B Asgeirsson, J G Schofield
Nov 1, 1985·Molecular and Cellular Endocrinology·D DelbekeP S Dannies
Apr 1, 1987·Molecular and Cellular Endocrinology·D BressionJ R Kiechel
Jan 1, 1984·Kroc Foundation Series·R Y Tsien
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D M ShobackE M Brown
Jan 1, 1990·Annals of the New York Academy of Sciences·M E Wolf, R H Roth
Jul 1, 1987·Journal of Neurochemistry·A B CubittP R Dobson
Aug 1, 1985·Clinical Endocrinology·B L BrownS Tomlinson
Jul 1, 1993·The American Journal of Physiology·C ChenJ D Vincent
Apr 1, 1991·The American Journal of Physiology·I S LoginR M MacLeod

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