PMID: 9630520Jun 19, 1998Paper

Use of acridine orange in: flow cytometric assessment of micronuclei induction

Mutation Research
K A CriswellM R Bleavins

Abstract

The micronucleus assay is a widely accepted method for evaluation of clastogens and aneugens. In the current study, acridine orange (AO) supravital staining was adapted for flow cytometric usage to assess micronucleated cells in rat bone marrow and spleen. Cyclophosphamide was used as a positive control test compound and results were compared to manual scoring in Wright-stained slides. In bone marrow, both manual and flow cytometric methods demonstrated positive dose response-trends for micronucleated polychromatic erythrocytes (MNPCE). Significant elevations in MNPCE were observed at all doses of cyclophosphamide, and comparisons between methods in bone marrow were not statistically different. The flow cytometric method was more sensitive in spleen samples, showing dose- and time-related increases in micronuclei compared with manual scoring. AO proved to be a sensitive discriminator of RNA and DNA, allowing distinct separation of polychromatic erythrocytes (PCE), normochromic erythrocytes (NCE), total nucleated cells (TNC), and micronucleated populations within both PCE and NCE regions. These results support the use of AO-based flow cytometry to provide a rapid and sensitive indicator of micronuclei inducers.

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Jun 19, 1998·Mutation Research·K A CriswellM R Bleavins

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Citations

Jan 18, 2011·Applied Biochemistry and Biotechnology·Kavindra Kumar KesariJitendra Behari
Sep 28, 2000·Mutation Research·G KrishnaJ Paulissen
Dec 13, 2000·Mutation Research·G Krishna, M Hayashi
Jul 23, 2003·Mutation Research·K A CriswellM R Bleavins
Jun 19, 1998·Mutation Research·K A CriswellM R Bleavins
Dec 18, 2010·Mutagenesis·Zbigniew DarzynkiewiczMichael Fenech
Feb 18, 2016·Journal of Agricultural and Food Chemistry·Sumit Kumar, Ashu Bhan Tiku
Jan 8, 2013·Mutation Research·Asako HaradaMasayuki Mishima
Jun 12, 2012·Ecotoxicology and Environmental Safety·Taynah VicariMarta Margarete Cestari
Oct 20, 2012·International Journal of Radiation Biology·Sanjay KumarRashmi Sisodia
Jul 7, 2001·Ecotoxicology and Environmental Safety·F Ayllón, E Garcia-Vazquez
Sep 6, 2011·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Ashok AgarwalKavindra Kesari
Jan 21, 2000·Journal of Applied Toxicology : JAT·K A CriswellM R Bleavins
Jul 27, 2001·Environmental and Molecular Mutagenesis·D K TorousC R Tometsko

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