Use of airway epithelial cell culture to unravel the pathogenesis and study treatment in obstructive airway diseases

Pulmonary Pharmacology & Therapeutics
Tinne C J MertensPieter S Hiemstra

Abstract

Asthma and chronic obstructive pulmonary disease (COPD) are considered as two distinct obstructive diseases. Both chronic diseases share a component of airway epithelial dysfunction. The airway epithelium is localized to deal with inhaled substances, and functions as a barrier preventing penetration of such substances into the body. In addition, the epithelium is involved in the regulation of both innate and adaptive immune responses following inhalation of particles, allergens and pathogens. Through triggering and inducing immune responses, airway epithelial cells contribute to the pathogenesis of both asthma and COPD. Various in vitro research models have been described to study airway epithelial cell dysfunction in asthma and COPD. However, various considerations and cautions have to be taken into account when designing such in vitro experiments. Epithelial features of asthma and COPD can be modelled by using a variety of disease-related invoking substances either alone or in combination, and by the use of primary cells isolated from patients. Differentiation is a hallmark of airway epithelial cells, and therefore models should include the ability of cells to differentiate, as can be achieved in air-liquid interface models. ...Continue Reading

References

May 10, 2016·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Takayasu NomuraShinji Saitoh
Jul 14, 2016·American Journal of Respiratory and Critical Care Medicine·Griet ConickxGuy G Brusselle
Nov 4, 2016·Journal of Biomedical Materials Research. Part a·Nathaniel J HogrebeKeith J Gooch
Dec 17, 2016·American Journal of Respiratory Cell and Molecular Biology·Martina GentzschScott H Randell

❮ Previous
Next ❯

Citations

Jan 19, 2019·Experimental Lung Research·Hong ZhangHongxia Zhao
Jan 31, 2018·In Vitro Cellular & Developmental Biology. Animal·Johnny L CarsonMilan J Hazucha
Oct 31, 2018·Respiratory Research·Dennis LoCynthia J Koziol-White
Jun 1, 2018·Applied in Vitro Toxicology·Samantha C Faber, Shaun D McCullough
Jul 11, 2020·Advanced Biosystems·Arbel Artzy-SchnirmanJosué Sznitman
Jul 3, 2019·Biomicrofluidics·Kaiyan LiXinghua Gao
Sep 14, 2019·The European Respiratory Journal·Pieter S HiemstraSam M Janes
Apr 26, 2018·Chinese Medical Journal·Gimano D Amatngalim, Pieter S Hiemstra
Sep 20, 2018·American Journal of Respiratory Cell and Molecular Biology·Joffrey MiannéJohn De Vos
Mar 7, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mi Ho JeongKyu Hyuck Chung
Sep 28, 2021·Molecular Microbiology·Flávia VianaGunnar N Schroeder

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.