Use of Ames test in evaluation of shale oil fractions

Environmental Health Perspectives
R A Pelroy, M R Petersen

Abstract

Conditions that affect the sensitivity of the Ames assay of complex hydrocarbon mixtures derived from shale oil were studied. Two fractions, one enriched in polynuclear aromatic compounds (PNA fraction), and a second fraction enriched in aromatic and heterocyclic amines (basic fraction), were selected for most of this work because of their comparatively high mutagenicity (i.e., compared with raw shale oil). The crude shale oil, as well as the basic, PNA, and tar fractions were mutagenic against the Salmonella typhimurium test strains, TA98 and TA100. Mutation was dependent on metabolic activation by microsomal (S9) enzymes. Both test strains responded equally well to the crude product and to the basic fraction; however, strain TA100 was more effective than TA198 in demonstrating the mutagenicity of the PNA fraction. The mutagenicity of the tar fraction could be most easily detected after metabolic activation in a liquid medium, as opposed to S9 activation in the top agar of the standard Ames assay. The mutagenicity of the basic fraction or 2-aminoanthracene was also demonstrated by metabolic activation in a liquid medium. In other set of experiments, the effect of chemical composition on the expression of mutagenicity in the st...Continue Reading

References

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Citations

Mar 1, 1984·Environmental Health Perspectives·B E Vaughan
Feb 1, 1992·Mutation Research·L NylundM Sorsa
Feb 1, 1982·Toxicology Letters·D WarshawskyG Moore
Jan 1, 1982·Cancer Letters·R I ChanW D Powrie
Aug 1, 1984·Journal of Applied Toxicology : JAT·J H CarverR W King
Dec 20, 2012·African Journal of Paediatric Surgery : AJPS·Adesoji O AdemuyiwaOlumide A Elebute
Jan 1, 1984·Environmental Mutagenesis·D M DeMariniA W Hsie
Apr 1, 1983·Journal of Toxicology and Environmental Health·M E Frazier, T K Andrews
Nov 1, 1981·Journal of Toxicology and Environmental Health·J A Ellenton, D J Hallett

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