Use of amides as cryoprotectants in extenders for frozen sperm of tambaqui, Colossoma macropomum

Theriogenology
A S Varela JuniorM R C Figueiredo

Abstract

Amides were tested as cryoprotectants in comparison with glycerol and DMSO (more traditional cryoprotectants) for recovery of Colossoma macropomum (tambaqui fish) sperm. Milt was extended in Beltsville Thawing Solution, then frozen with the addition of 2%, 5%, 8%, or 11% of: (1) dimethylacetamide (DMA), (2) dimethylformamide (DMF), (3) methylformamide (MF), or with 5% glycerol or 10% dimethylsulfoxide. Fertilization rates were greatest (P<0.001) with amides; 8% DMF (91.6±1.3%), 5% DMF (88.9±1.6%), and 8% MF (83.0±1.6%), which did not significantly differ among themselves, when compared with glycerol (51.6±2.4%) and DMSO (61.9±3.1%). The best hatching rates (P<0.001) also occurred for 5% or 8% DMF and 8% MF (79.1±3.1, 87.6±1.5, and 74.8±3.0, respectively) and were also similar (P>0.05). For such treatments, both fertilization and hatching rates were similar (P>0.05) to those with fresh sperm (91.7±1.4 and 87.4±1.4, respectively). The best sperm motility across extenders (at least 55.7%) was with 5%, 8%, and 11% DMF (P<0.001). Those same treatments, along with 11% MF, provided the longest (P<0.001) period of motility (at least 1 min). The greatest sperm integrity (more than 54%) was with 5% and 11% MF and with DMA and DMF at all ...Continue Reading

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Citations

Aug 5, 2014·Aquatic Toxicology·Fernanda Moreira LopesCarlos Eduardo da Rosa
Sep 17, 2013·Aquatic Toxicology·Cyntia Ayumi Yokota HarayashikiCarine Dahl Corcini
Sep 8, 2015·Fish Physiology and Biochemistry·Janaína Camacho da SilvaCarine Dahl Corcini
Apr 25, 2015·Animal Reproduction Science·A S Varela JuniorC D Corcini
Mar 1, 2015·Cryobiology·Alexandre Nizio MariaHymerson Costa Azevedo
May 20, 2018·Fish Physiology and Biochemistry·Carine de Freitas SouzaBernardo Baldisserotto
Dec 5, 2018·Bulletin of Environmental Contamination and Toxicology·Tony SilveiraVinicius Farias Campos
Jul 15, 2020·Molecular Biology Reports·Larissa O DaneluzVinicius F Campos
Nov 30, 2019·Brazilian Journal of Biology = Revista Brasleira De Biologia·J M GaloR P Ribeiro
Jan 26, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Cecília Bertacini de AssisJoão Paulo Silva Pinheiro
Apr 2, 2021·Animal Reproduction·Gabriel Felipe Oliveira de MenezesAntonio de Lisboa Ribeiro

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