Use of an Electrochemical Split Cell Technique to Evaluate the Influence of Shewanella oneidensis Activities on Corrosion of Carbon Steel

PloS One
Robert Bertram MillerChelsea N Monty

Abstract

Microbially induced corrosion (MIC) is a complex problem that affects various industries. Several techniques have been developed to monitor corrosion and elucidate corrosion mechanisms, including microbiological processes that induce metal deterioration. We used zero resistance ammetry (ZRA) in a split chamber configuration to evaluate the effects of the facultatively anaerobic Fe(III) reducing bacterium Shewanella oneidensis MR-1 on the corrosion of UNS G10180 carbon steel. We show that activities of S. oneidensis inhibit corrosion of steel with which that organism has direct contact. However, when a carbon steel coupon in contact with S. oneidensis was electrically connected to a second coupon that was free of biofilm (in separate chambers of the split chamber assembly), ZRA-based measurements indicated that current moved from the S. oneidensis-containing chamber to the cell-free chamber. This electron transfer enhanced the O2 reduction reaction on the coupon deployed in the cell free chamber, and consequently, enhanced oxidation and corrosion of that electrode. Our results illustrate a novel mechanism for MIC in cases where metal surfaces are heterogeneously covered by biofilms.

References

Oct 10, 1998·Research in Microbiology·Z DzierzewiczT Wilczok
Mar 2, 2002·Applied and Environmental Microbiology·M DubielD K Newman
May 8, 2003·Applied Microbiology and Biotechnology·A K Lee, D K Newman
Jun 15, 2004·Current Opinion in Biotechnology·Iwona B Beech, Jan Sunner
Nov 18, 2004·Journal of Bacteriology·Kai M ThormannAlfred M Spormann
Jan 22, 2005·Journal of Bacteriology·Kai M ThormannAlfred M Spormann
Jul 24, 2007·Applied and Environmental Microbiology·Orianna BretschgerKenneth H Nealson
Aug 19, 2007·Applied Microbiology and Biotechnology·Rongjun Zuo
Aug 8, 2009·The Journal of Biological Chemistry·Daniel BaronDaniel R Bond
Dec 10, 2013·Applied and Environmental Microbiology·Dennis Enning, Julia Garrelfs
May 13, 2014·Journal of the Science of Food and Agriculture·Kieran JordanMindaugas Malakauskas
Dec 10, 2014·Best Practice & Research. Clinical Rheumatology·Mark AsquithJames T Rosenbaum
Apr 8, 2015·Journal of Basic Microbiology·Johnson Lin, Bafana B Madida

❮ Previous
Next ❯

Citations

Apr 15, 2018·Applied and Environmental Microbiology·Robert B MillerJohn M Senko
Jul 25, 2020·Scientific Reports·Silvia J Salgar-ChaparroLaura L Machuca

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
electron exchange

Software Mentioned

ZRA

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.