PMID: 9431926Feb 12, 1998Paper

Use of an enrichment broth cultivation-PCR combination assay for rapid diagnosis of swine erysipelas

Journal of Clinical Microbiology
Y ShimojiY Yokomizo

Abstract

We have previously described the creation by Tn916 mutagenesis of avirulent transposition mutants from a highly virulent strain of Erysipelothrix rhusiopathiae, the causative agent of swine erysipelas. In this study, we cloned a 2.2-kb DNA fragment which flanked the Tn916 insertion in an avirulent mutant (strain 33H6) and evaluated the possibility that this region could be used for the specific detection of E. rhusiopathiae. According to the sequences of this region, oligonucleotide primers were designed to amplify a 937-bp fragment of the E. rhusiopathiae chromosome by PCR. The specificity of the PCR was investigated by analyzing 64 strains of Erysipelothrix species and 27 strains of other genera different from Erysipelothrix. A 937-bp DNA fragment could be amplified from all E. rhusiopathiae strains tested, and no amplification was observed by using DNAs from the other species tested. To make a rapid and definite diagnosis of swine erysipelas in slaughterhouses, we developed an enrichment broth cultivation-PCR combination assay, which used a commercially available DNA extraction kit, to identify E. rhusiopathiae in the specimens from swine with arthritis. After samples were enriched in selective broth culture, detection of E....Continue Reading

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Citations

Oct 14, 2004·Journal of Applied Microbiology·H N ChinivasagamP J Blackall
May 2, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kazuki HaradaToshio Takahashi
Sep 10, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ho ToTetsuo Nunoya
Sep 30, 2015·Acta Veterinaria Scandinavica·Pernille Engelsen EtterlinStina Ekman
Sep 15, 2011·Tropical Animal Health and Production·Tania Alen CoutinhoJosé Soares Ferreira Neto
Aug 23, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Yoshinao Yamazaki
Mar 26, 2014·Avian Pathology : Journal of the W.V.P.A·Helena ErikssonDésirée S Jansson
Sep 15, 2020·Journal of Medical Microbiology·Yudai TaguchiYoshihiro Shimoji
Aug 3, 2019·Microbiology and Immunology·Yoshihiro ShimojiToshiyuki Tsutsui
Oct 30, 2001·Journal of Clinical Microbiology·A T OkataniH Hayashidani
Dec 2, 2000·Journal of Clinical Microbiology·A T OkataniK Kaneko
Aug 26, 2003·Veterinary Microbiology·Raafat HassaneinYoshikazu Suzuki
May 3, 2005·Veterinary Microbiology·Qinning WangThomas V Riley
Oct 19, 2010·Journal of Microbiological Methods·Tania Alen CoutinhoAndrea Micke Moreno
Jan 22, 2011·Diagnostic Microbiology and Infectious Disease·Tania A CoutinhoAndrea M Moreno
Sep 8, 2009·Veterinary Microbiology·Qinning WangThomas V Riley

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