Use of an in vitro model of tissue-engineered human skin to study keratinocyte attachment and migration in the process of reepithelialization

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Caroline A HarrisonS Mac Neil

Abstract

To produce a stable epidermis, keratinocytes need to be firmly attached to the basement membrane. However, following wounding, keratinocytes are required to develop a migratory phenotype in order to reepithelialize the wound. To investigate some of the issues underlying reepithelialization, we have developed a three-dimensional in vitro model of tissue-engineered skin, comprising sterilized human dermis seeded with human keratinocytes and dermal fibroblasts. Using this model, we have shown that the inclusion of fibroblasts within the model increases the stability of keratinocyte attachment. We have also demonstrated that keratinocyte migration occurs most effectively in the absence of a basement membrane and following the inclusion of fibroblasts in the model. In addition, subjecting the keratinocyte layer to mechanical trauma induces a migratory phenotype. We conclude that this three-dimensional in vitro wound model can be used to increase our understanding of the factors that enhance keratinocyte migration and hence wound healing in vivo.

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Citations

Apr 9, 2009·Archives of Dermatological Research·James VaraniJohn J Voorhees
Oct 30, 2009·Journal of the Royal Society, Interface·Rostislav V ShevchenkoS Elizabeth James
Oct 26, 2007·The Journal of Investigative Dermatology·Eve E KandybaPatricia E Martin
Jan 30, 2008·Burns : Journal of the International Society for Burn Injuries·Caroline A Harrison, Sheila MacNeil
Feb 13, 2007·European Journal of Cell Biology·Karsten BoehnkeHans-Jürgen Stark
Sep 8, 2015·European Journal of Cell Biology·Gopu SriramMei Bigliardi-Qi
Nov 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Catherine A HernonSheila MacNeil
Nov 17, 2007·Journal of Anatomy·Heiko SorgBrigitte Vollmar
Jan 17, 2007·The British Journal of Dermatology·C A HarrisonS MacNeil
Feb 6, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nadja Nicole ZöllerAugust Bernd
Nov 25, 2011·Burns : Journal of the International Society for Burn Injuries·Matti PeuraJyrki Vuola
Mar 21, 2008·Journal of Burn Care & Research : Official Publication of the American Burn Association·Daniel J A ThorntonSheila MacNeil
Jul 7, 2010·Journal of Tissue Engineering and Regenerative Medicine·Louise E SmithSheila MacNeil

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