PMID: 8960135Dec 2, 1996Paper

Use of an infectious Simian virus 40-based shuttle vector to analyse UV-induced mutagenesis in monkey cells

Mutation Research
C HeraA Sarasin

Abstract

SV40 based shuttle vectors able to be packaged as pseudovirions have been used either as naked DNA or as pseudovirus to analyse the mutation frequency and the UV-induced mutation spectra obtained after transfection or infection of COS7 monkey cells. The frequency of supF spontaneous mutants was similar whatever the state of the vector, indicating that the transfection step is not responsible for the high spontaneous mutation frequency when using shuttle vectors. Nevertheless the UV-induced mutation frequency of the supF gene was higher when transfected DNA was replicated into COS7 cells than when pseudovirus infection was performed. The UV induced mutation spectra was basically similar in both situations but a new hot-spot at nucleotide 110 was obtained after pseudovirus infection. UV-pretreated and control COS7 cells were infected with untreated or UV-damaged pi SVPC7 shuttle virus and the survival and the supF mutation frequency were analysed in the progeny. The survival of UV-damaged pseudovirus replicated in 10 J/m2 UV-pretreated cells was 2-fold higher than in untreated cells. This increase in the survival was accompanied by a slight enhancement in the number of supF mutants.

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Aug 1, 1992·Molecular & General Genetics : MGG·J J BoesenJ W Simons
Mar 1, 1990·Photochemistry and Photobiology·P Di MascioH Sies
Mar 1, 1989·Mutation Research·C F MenckA Sarasin
Jan 1, 1985·Cancer Investigation·A Sarasin
Jul 1, 1988·Somatic Cell and Molecular Genetics·M ProtićK Dixon
Jan 1, 1988·Mutagenesis·R B DuBridge, M P Calos
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A BredbergM M Seidman
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E A DrobetskyB W Glickman
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A RazzaqueM M Seidman
Sep 1, 1983·Nature·F Bourre, A Sarasin
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M P CalosM R Botchan
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C N Parris, K H Kraemer

❮ Previous
Next ❯

Citations

Jun 6, 2000·Mutation Research·K A Canella, M M Seidman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.