PMID: 43387Dec 1, 1979

Use of an iodide-specific electrode to study lactoperoxidase-catalyzed iodination of l-tyrosine

Journal of Pharmaceutical Sciences
R M ThreatteP K Jones


An in vitro method employing an iodide-specific electrode for monitoring lactoperoxidase-catalyzed iodination is described. The method utilized lactoperoxidase, potassium iodide, and a glucose--glucose oxidase system for the generation of hydrogen peroxide and l-tyrosine. As iodination of l-tyrosine proceeded, the free iodide concentration in solution decreased and was monitored by an iodide-specific electrode. The iodide electrode was reliable when compared to a 131I-method for measuring free iodide changes in solution. Increasing concentrations of resorcinol, a well-known inhibitor of thyroid peroxidase-catalyzed iodination, in the reaction mixture resulted in graded inhibition of the initial rate of lactoperoxidase-catalyzed l-tyrosine iodination. This in vitro system can be used to assess inhibitory activity of various antithyroid substances.


Mar 1, 1971·Immunochemistry·M MorrisonR G Webster
Jul 21, 1970·Biochemistry·M Morrison, G S Bayse
Mar 22, 1967·Biochimica Et Biophysica Acta·L J DEGROOT
Apr 1, 1965·Endocrinology·L J DEGROOTA D DUNN

Related Concepts

Iodine Radioisotopes
Hydrogen-Ion Concentration

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