Use of an ipb-lux Fusion To Study Regulation of the Isopropylbenzene Catabolism Operon of Pseudomonas putida RE204 and To Detect Hydrophobic Pollutants in the Environment.

Applied and Environmental Microbiology
O V Selifonova, R W Eaton

Abstract

A DNA segment involved in the regulation of the isopropylbenzene (cumene) catabolism operon (ipb) of plasmid pRE4 from Pseudomonas putida RE204 and the Vibrio fischeri luciferase genes, luxCDABE, were used to create an ipbRo/pA(prm1)-luxCDABE reporter fusion plasmid, pOS25. Escherichia coli HMS174(pOS25) produces light in the presence of inducers of the ipb operon. These inducers were shown to be hydrophobic compounds and to include monoalkylbenzenes, substituted benzenes and toluenes, some alkanes and cycloalkanes, chlorinated solvents, and naphthalenes. Complex hydrocarbon mixtures, such as gasoline, diesel fuel, jet fuels (JP-4 and JP-5), and creosote, were also inducers of ipb-lux. Bacteria carrying the ipb-lux reporter may be useful as bioindicators of hydrocarbon pollution in the environment and may be particularly valuable for examining the bioavailability of inducing pollutants.

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J L CampbellF W Studier
Mar 1, 1985·Microbiological Reviews·H Nikaido, M Vaara
Nov 1, 1987·Journal of Bacteriology·P M RogowskyC I Kado
Jul 1, 1982·Journal of Bacteriology·R W Eaton, D W Ribbons
Jun 1, 1995·Microbiological Reviews·J SikkemaB Poolman
Dec 1, 1994·Applied and Environmental Microbiology·S Heald, R O Jenkins
May 1, 1994·Applied and Environmental Microbiology·T K Van DykR A LaRossa
Jul 1, 1994·Journal of Bacteriology·R W Eaton, J D Nitterauer
Sep 1, 1993·Applied and Environmental Microbiology·O SelifonovaT Barkay

❮ Previous
Next ❯

Citations

Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·E DíazJ L García
Jan 9, 2004·Applied and Environmental Microbiology·Christoph WerlenJan Roelof van der Meer
Jun 2, 2010·Nature Reviews. Microbiology·Jan Roelof van der Meer, Shimshon Belkin
Feb 6, 2020·FEMS Microbiology Reviews·Bruno MaucourtFrançoise Bringel
Nov 30, 2015·Applied Microbiology and Biotechnology·Wei HeChuan-Chao Dai
Feb 7, 2002·Journal of Microbiological Methods·Angela KeanePeter C K Lau
Jun 1, 1996·Current Opinion in Biotechnology·L P Wackett
Oct 18, 2001·Current Opinion in Biotechnology·G S SaylerD E Nivens
Nov 15, 1997·Applied and Environmental Microbiology·T BarkayR R Turner

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.