PMID: 11925395Apr 2, 2002Paper

Use of antisperm antibodies in differential display Western blotting to identify sperm proteins important in fertility

Human Reproduction
W W C Chiu, L W Chamley

Abstract

Antisperm antibodies (ASA) may be an important cause of infertility but current tests for the detection of ASA have poor prognostic value. The inadequacy of current tests may reflect the inability of these tests to define the antigenic specificity of the sperm proteins with which the ASA react. Identification of the sperm proteins that ASA bind to is a necessary preliminary step to the development of more useful diagnostic tests for ASA. A sensitive Western blotting technique was used to compare the antigenic specificities of ASA from men who were infertile (n = 6) with those who were fertile following vasectomy reversal (n = 3). Normal fertile men (n = 3) and infertile men with known ASA (n = 4) were also included in the analysis. All men, including the normal fertile controls, had ASA detectable in our system. Several sperm proteins were identified that react with ASA from infertile but not fertile men. Quantitative differences in the binding of ASA to some proteins were also demonstrated. Additionally, we demonstrated that normal motile sperm are coated with an antibody that appears to be bound to sperm by a non-antigenic mechanism. Sera from all men contained ASA, but clearly some of these did not cause infertility. Charact...Continue Reading

Citations

Jan 9, 2003·American Journal of Reproductive Immunology : AJRI·W W C Chiu, L W Chamley
Mar 12, 2004·Reproductive Biology and Endocrinology : RB&E·Anil Suri
Dec 10, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jing HuangXiaoyan Qiu
Feb 24, 2006·Asian Journal of Andrology·Giorgio Cavallini
Jul 12, 2007·Seminars in Immunopathology·L W Chamley, G N Clarke
May 30, 2014·Human Fertility : Journal of the British Fertility Society·Deborah PrendergastAndrew N Shelling

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