Use of bacteriocin-producing, probiotic strain Enterococcus faecium AL41 to control intestinal microbiota in farm ostriches

Letters in Applied Microbiology
A LaukováJ Ščerbová

Abstract

Probiotic enterococci can produce bacteriocins. Enterococcus faecium AL41 is an Enterocin M-producing, probiotic strain which has previously shown beneficial effect in broiler chickens. In this study, it was used to control intestinal microbiota in farm ostriches in a 42-day experiment with an experimental group (EG, 40 ostriches) and a control group (CG, 46). In addition to feed mixture, the ostriches in EG received Ent. faecium AL41 (10(9) CFU ml(-1); by rifampicin-marked variant) 400 μl per animal per day in their drinking water for 21 days. Sampling was carried out at the start of the experiment (at day 0/1), at day 21 (after 21 days of AL41 application) and at day 42 (21 days after AL41 cessation). Faeces (mixture, n = 6) were treated using the standard microbiological dilution method and cultivated on selective media (ISO). The highest count of AL41 was found at day 42. Its identity was confirmed with PCR and Maldi-Tof. The ostriches were free of Salmonella and Campylobacter cells. At day 21, antimicrobial effect was demonstrated by significant reduction in coagulase-positive and negative staphylococci in EG compared to CG (P < 0·001) and coliforms, Enterobacteria and Pseudomonas-like bacteria (P < 0·001). We conclude tha...Continue Reading

References

Sep 29, 1998·Letters in Applied Microbiology·A LaukováM Mareková
Dec 22, 2004·Veterinary Research Communications·A LaukováA Ouwehand
Jun 7, 2007·Journal of Industrial Microbiology & Biotechnology·Mária MarekováIngolf Nes
Dec 1, 2012·Probiotics and Antimicrobial Proteins·Andrea LaukováL'ubomír Ondruška

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Citations

Sep 8, 2016·Foodborne Pathogens and Disease·Jana Ščerbová, Andrea Lauková
Mar 28, 2017·Probiotics and Antimicrobial Proteins·Andrea LaukováJana Ščerbová
Mar 15, 2018·Avian Pathology : Journal of the W.V.P.A·Breno C B BeirãoLuiz Felipe Caron
Feb 9, 2018·Probiotics and Antimicrobial Proteins·Andrea LaukováViola Strompfová
May 8, 2020·Probiotics and Antimicrobial Proteins·Andrea LaukováMonika Pogány Simonová
Jan 7, 2022·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Andrea LaukováMonika Pogány Simonová

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