PMID: 9555887Apr 29, 1998Paper

Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli

Journal of Bacteriology
K C Murphy

Abstract

Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The deltarecBCD::Plac-red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable.

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Citations

Oct 15, 1998·Nature Genetics·Y ZhangA F Stewart
Mar 29, 2000·Journal of Bacteriology·A R Poteete, A C Fenton
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