Use of baculovirus MHC/peptide display libraries to characterize T-cell receptor ligands

Immunological Reviews
Frances CrawfordJohn W Kappler

Abstract

Peptide/protein display libraries are powerful tools for identifying and manipulating receptor/ligand pairs. While the large size of bacterial phage display libraries has made them the platform of choice in many applications, often considerable engineering has been required to achieve display of properly folded and active eukaryotic proteins, such as antibodies. This problem has been partially solved in several eukaryotic display systems, e.g. using yeast or retroviruses, but these systems have their own limitations. Recently, baculovirus has been developed as a display system using the virus itself or infected insect cells as the display platform. Here, we review the development and use of baculovirus-infected cells as a platform for display libraries of peptides bound to major histocompatibility complex (MHC) class I (MHCI) or class II (MHCII). We have used fluorescent multimeric soluble T-cell receptors (TCRs) to screen these libraries and to identify peptide antigen mimotopes. We also present some improvements to this system that allow very large libraries to be constructed and screened. We have used these libraries to examine the role of MHCII-bound peptides in the presentation of the staphylococcal enterotoxin A (SEA) and...Continue Reading

References

Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A HermanR P Sekaly
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·A Y HuangE M Jaffee
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·G K BalendiranJ C Sacchettini
Mar 26, 1998·Protein Engineering·M C KiekeK D Wittrup
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·C P LiuJ Kappler
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·B AndersonP Santamaria
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·W ReesJ Kappler
Feb 28, 2002·Annual Review of Immunology·Nilabh ShastriThomas Serwold
Mar 8, 2002·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·E SimpsonP J Dyson
Jun 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xinqi LiuJohn Kappler
Jul 24, 2002·Journal of Immunological Methods·Gordon Tribbick
Nov 26, 2002·Immunological Reviews·Pierre Van Der BruggenThierry Boon
Dec 14, 2002·Trends in Biotechnology·Sang Yup LeeZhaohui Xu
Dec 31, 2002·Journal of Immunological Methods·Susan E BrophyDavid M Kranz
Mar 14, 2003·Trends in Biochemical Sciences·Terry T TakahashiRichard W Roberts
Apr 5, 2003·Protein Engineering·Scott E StarwaltDavid M Kranz
Apr 18, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Roland Wolkowicz, Garry P Nolan
May 3, 2003·Methods : a Companion to Methods in Enzymology·Mireia SospedraRoland Martin
Jun 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Scott M LiebermanTeresa P DiLorenzo
Sep 23, 2003·Nucleic Acids Research·Masato YonezawaHiroshi Yanagawa
Nov 6, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sari P KukkonenSeppo Ylä-Herttuala
Dec 4, 2003·Nature Reviews. Immunology·Jonathan W YewdellJacques Neefjes
Feb 3, 2004·Journal of Receptor and Signal Transduction Research·Michael Szardenings
Apr 20, 2004·PLoS Biology·Frances CrawfordJohn W Kappler
Oct 1, 1993·Current Biology : CB·E K O'SheaP S Kim
Dec 8, 2004·Current Protein & Peptide Science·Alessandro PiniLuisa Bracci
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yibing WangJohn W Kappler
Jul 30, 2005·Cell·Eric S HusebyJohn W Kappler

❮ Previous
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Citations

Sep 1, 2012·Immunologic Research·Jonathan D Buhrman, Jill E Slansky
Feb 9, 2010·Nature Immunology·Brian D StadinskiKathryn Haskins
Nov 17, 2007·Nature Reviews. Immunology·Nathan J Felix, Paul M Allen
Jul 16, 2011·Nature Reviews. Immunology·Mark M DavisEvan W Newell
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kimberly R JordanJill E Slansky
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Brian D StadinskiJohn W Kappler
Sep 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Frances CrawfordJohn W Kappler
Oct 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lei YinShaodong Dai
Oct 10, 2013·The Journal of Biological Chemistry·Jonathan D BuhrmanJill E Slansky
Jul 15, 2011·Protein Engineering, Design & Selection : PEDS·Fei WenHuimin Zhao
Aug 14, 2008·BMC Biotechnology·Helene Y Meller HarelPaul A Millner
Nov 1, 2013·Genome Medicine·Daniel J WoodsworthRobert A Holt
Aug 9, 2007·Proceedings of the American Thoracic Society·Philippa Marrack
Jan 21, 2014·Nature Biotechnology·Evan W Newell, Mark M Davis
Apr 24, 2014·Human Immunology·Govinda Sharma, Robert A Holt
Jul 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Wei Jiang, Eric T Boder
May 14, 2014·Trends in Immunology·Brian D Stadinski, Eric S Huseby
Jan 1, 2011·Annual Review of Chemical and Biomolecular Engineering·Eric T Boder, Wei Jiang
Apr 2, 2008·Trends in Immunology·Julia K ArchboldJames McCluskey
Oct 11, 2012·Immunological Reviews·Michael E BirnbaumK Christopher Garcia
Nov 10, 2011·Diabetes/metabolism Research and Reviews·Li ZhangGeorge S Eisenbarth
Apr 24, 2015·Cytotechnology·Shinya TakadaHideki Yamaji
May 27, 2014·Cell·Michael E BirnbaumK Christopher Garcia
Jan 13, 2010·Immunity·Whitney A MacdonaldJames McCluskey
Apr 7, 2009·Current Protocols in Immunology·Kimberly R JordanJill E Slansky
Jan 2, 2020·International Journal of Molecular Sciences·Krištof Bozovičar, Tomaž Bratkovič
Jul 8, 2020·Nature Methods·Alok V Joglekar, Guideng Li
Dec 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kimberly R JordanJill E Slansky
Oct 24, 2020·The Journal of Experimental Medicine·Brendan ReedJohn W Kappler
Feb 12, 2021·Frontiers in Immunology·Heather F JonesDavid A Scheinberg
Jun 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Pengcheng WeiLei Yin
Oct 22, 2021·FEMS Microbiology Reviews·Weronika JaroszewiczGrzegorz Węgrzyn

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