Use of benzoate as an electron acceptor by Syntrophus aciditrophicus grown in pure culture with crotonate

Environmental Microbiology
Housna MouttakiM J McInerney

Abstract

In methanogenic environments, the main fate of benzoate is its oxidization to acetate, H(2) and CO(2) by syntrophic associations of hydrogen-producing benzoate degraders and hydrogen-using methanogens. Here, we report the use of benzoate as an electron acceptor. Pure cultures of S. aciditrophicus simultaneously degraded crotonate and benzoate when both substrates were present. The growth rate was 0.007 h(-1) with crotonate and benzoate present compared with 0.025 h(-1) with crotonate alone. After 8 days of incubation, 4.12 +/- 0.50 mM of cyclohexane carboxylate and 8.40 +/- 0.61 mM of acetate were formed and 4.0 +/- 0.04 mM of benzoate and 4.8 +/- 0.5 mM of crotonate were consumed. The molar growth yield was 22.7 +/- 2.1 g (dry wt) of cells per mol of crotonate compared with about 14.0 +/- 0.1 g (dry wt) of cells per mol of crotonate when S. aciditrophicus was grown with crotonate alone. Cultures grown with [ring-(13)C]-benzoate and unlabelled crotonate initially formed [ring-(13)C]-labelled cyclohexane carboxylate. No (13)C-labelled acetate was detected. In addition to cyclohexane carboxylate, (13)C-labelled cyclohex-1-ene carboxylate was detected as an intermediate. Once almost all of the benzoate was gone, carbon isotopic an...Continue Reading

References

Oct 1, 1979·Biochemical Society Transactions·M T BalbaW C Evans
Mar 1, 1977·Bacteriological Reviews·R K ThauerK Decker
Feb 1, 1976·Archives of Microbiology·J G Ferry, R S Wolfe
Jan 1, 1987·Annual Review of Microbiology·T K Kirk, R L Farrell
Jun 11, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·H Dalton, D I Stirling
Oct 1, 1980·Biochemical Society Transactions·M T Balba, W C Evans
May 18, 2000·Archives of Microbiology·J A Müller, B Schink
Jul 27, 2002·Annual Review of Microbiology·Jane Gibson, Caroline S Harwood
Nov 5, 2002·Current Opinion in Chemical Biology·Matthias BollJohann Heider
Feb 1, 1980·Applied and Environmental Microbiology·J B HealyM Reinhard
Aug 1, 1985·Applied and Environmental Microbiology·D Grbić-Galić, L Y Young
Jun 1, 1994·Applied and Environmental Microbiology·A RamosH Santos
Dec 13, 2006·Applied and Environmental Microbiology·Housna MouttakiMichael J McInerney

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Citations

Dec 31, 2008·Applied and Environmental Microbiology·Housna MouttakiMichael J McInerney
Feb 5, 2013·Journal of Bacteriology·Marie KimWolfgang Buckel
Jul 19, 2012·Annual Review of Microbiology·Jessica R SieberRobert P Gunsalus
Nov 10, 2009·Current Opinion in Biotechnology·Michael J McInerneyRobert P Gunsalus
Aug 13, 2015·Environmental Science and Pollution Research International·Wei GuanShuguang Xie
Jan 7, 2014·Environmental Microbiology·Jessica R SieberMichael J McInerney
Aug 13, 2014·Journal of Bacteriology·Johannes W KungMatthias Boll
Feb 26, 2019·Frontiers in Microbiology·Juan J L GuzmanLargus T Angenent
Sep 30, 2021·Proceedings of the National Academy of Sciences of the United States of America·Michael AgneMatthias Boll

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