PMID: 2118658Sep 1, 1990

Use of brefeldin A to define sites of glycosphingolipid synthesis: GA2/GM2/GD2 synthase is trans to the brefeldin A block

Proceedings of the National Academy of Sciences of the United States of America
W W YoungS Lechler-Osborn

Abstract

Brefeldin A (BFA) induces the rapid redistribution of the Golgi complex into the endoplasmic reticulum (ER), causing the glycoproteins that are retained in the ER to be processed by Golgi enzymes. We have examined the effects of BFA on the synthesis of glycosphingolipids (GSL) to map the intracellular sites of GSL synthesis. In several cultured cell types, BFA inhibited the synthesis of the neutral GSL gangliotriaosylceramide (GA2) and monosialoganglioside GM2 and disialoganglioside GD2, where GD2 is GalNAc(beta 1----4)- [NeuAc(alpha 2----8)NeuAc(alpha 2----3)]Gal(beta 1----4)GlcCer, GM2 lacks the NeuAc(alpha 2----8) unit, and GA2 lacks both NeuAc(alpha 2----8) and NeuAc(alpha 2----3) units. The observed decrease in labeling of GA2, GM2, and GD2 in the presence of BFA was not due either to enhanced degradation of these glycolipids or to shedding of these glycolipids from the cells. In rat liver all three of these glycolipids have been shown by others to be synthesized by the same enzyme, GA2/GM2/GD2 synthase, which catalyzes the addition of N-acetylgalactosamine to lactosylceramide (Lac-Cer), GM3 [NeuAc(alpha 2----3)Gal(beta 1----4)GlcCer], and GD3 [NeuAc(alpha 2----8)NeuAc-(alpha 2----3)Gal(beta 1----4)GlcCer], respectively. S...Continue Reading

References

Apr 20, 1994·Biochimica Et Biophysica Acta·K J KallenP Quinn
Aug 4, 1994·Biochimica Et Biophysica Acta·P J Trotter, D R Voelker
Jan 1, 1993·Progress in Lipid Research·D Allan, K J Kallen
Dec 11, 1992·Biochimica Et Biophysica Acta·D Hoekstra, Jan Willem Kok
Jun 13, 1998·Progress in Lipid Research·L RiboniG Tettamanti
Jun 17, 2000·Biochimica Et Biophysica Acta·G Van Meer, J C Holthuis
Mar 5, 1999·Biochimica Et Biophysica Acta·H J MaccioniJ A Martina
Sep 8, 1999·The Biochemical Journal·C G GiraudoH J Maccioni
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C ZuberK Moremen
Feb 1, 1991·The Journal of Cell Biology·J Lippincott-SchwartzR D Klausner
Dec 1, 1991·The Journal of Cell Biology·R C SpiroJ A Garcia
Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Dec 14, 2011·Human Molecular Genetics·Elise RoyJean Michel Heard
Aug 20, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Feng GuanSen-Itiroh Hakomori
Nov 1, 1995·Neurochemical Research·S U WalkleyK Dobrenis
Mar 24, 1999·Molecular Membrane Biology·D J Sillence, D Allan
Mar 23, 2011·FEBS Letters·H J MaccioniWaldo Spessott
Dec 6, 2007·Journal of Neurochemistry·H J Maccioni
Aug 1, 1993·Current Opinion in Cell Biology·G Van Meer
Jun 24, 2010·Analytical Biochemistry·Tomohisa KatoKenichi Hatanaka
Dec 1, 1991·Biochemical Genetics·A M Novikov, T N Seyfried
Apr 14, 2016·Glycobiology·Chin Fen TeoLance Wells
Dec 21, 1992·FEBS Letters·P H Fishman, P K Curran
Aug 3, 2000·FEBS Letters·F SadeghlarG van Echten-Deckert
Aug 6, 2003·The Journal of Biological Chemistry·Claudio G Giraudo, H J Maccioni
Aug 10, 2007·Nature·Giovanni D'AngeloMaria Antonietta De Matteis
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Robert Jan VeldmanJan Willem Kok
Oct 3, 2001·Physiological Reviews·J C HolthuisG Van Meer

Citations

Feb 1, 1989·The Journal of Cell Biology·G GriffithsK Simons
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J B Ulmer, G E Palade
Jan 1, 1989·Journal of Neurochemistry·G van Echten, K Sandhoff
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G PohlentzK Sandhoff
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P H FishmanG J Todaro
Feb 27, 1974·Biochemical and Biophysical Research Communications·G YogeeswaranJ A Wright
Mar 15, 1982·International Journal of Cancer. Journal International Du Cancer·M Y YehK E Hellström
Jul 1, 1980·Journal of Neurochemistry·M A Williams, R H McCluer

Related Concepts

Glycosphingolipids
Golgi Apparatus
N-Acetylgalactosaminyltransferases
WBSCR17 protein, mouse
Carbohydrate Linkage
Carbohydrate Sequence
Galactosyltransferases
Brefeldin A
Antibiotics
Melanoma

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