Use of CA15‑3 for screening breast cancer: An antibody‑lectin sandwich assay for detecting glycosylation of CA15‑3 in sera

Oncology Reports
Jae Woong ChoiHong-Jin Kim

Abstract

Elevated serum CA15‑3 assessed by enzyme‑linked immunosorbent assay (ELISA) has been considered a diagnostic marker of breast cancer. However, accumulating data indicate that the current ELISA system for detecting CA15‑3, which targets the peptide backbone of CA15‑3, is not sufficiently sensitive to detect early or localized breast cancer. In the present study, we designed an antibody‑lectin sandwich assay detecting glycosylation of CA15‑3 in patients with breast cancer. Ιmmobilized anti‑CA15‑3 monoclonal antibody captures CA15‑3 in serum, and glycosylation of the CA15‑3 is detected with Concanavalin A (ConA) lectin, which preferentially bind high‑mannose N‑glycans. ConA provided the best signal for detecting serum CA15‑3 among 9 types of lectin, Since CA15‑3 is a heavily glycosylated protein, detecting the glycosylation of CA15‑3 should be a much more sensitive way to assess CA15‑3 than the current ELISA method. Linear responses were obtained in the anti‑CA15‑3 antibody‑ConA sandwich assay when sera were diluted up to 2000‑fold. This dilution factor is comparable with that of the current ELISA system which allows 50‑ to 100‑fold serum dilutions. The glycosylation level of CA15‑3 was found to increase with increasing breast can...Continue Reading

Citations

Feb 23, 2020·The International Journal of Neuroscience·Xuemin ChengZhijian Liang
Oct 24, 2019·Current Molecular Medicine·Zsuzsanna ElekZsolt Rónai
Oct 1, 2020·Cancers·Jiawei LiDong-Xu Liu
Jul 10, 2019·ACS Chemical Biology·Katarzyna HermanArkadiusz Ptak
Mar 18, 2020·Journal of Proteome Research·Marketa LastovickovaJanette Bobalova

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Methods Mentioned

BETA
biopsy
enzyme-linked immunosorbent assay
ELISA
glycosylation
electrophoresis
X-ray
sandwich

Software Mentioned

GraphPad prism
GraphPad
Power

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