Use of CDP-glycerol as an alternate acceptor for the teichoic acid polymerase reveals that membrane association regulates polymer length.

Journal of Bacteriology
Jeffrey W Schertzer, Eric D Brown

Abstract

The study of bacterial extracellular polysaccharide biosynthesis is hampered by the fact that these molecules are synthesized on membrane-resident carrier lipids. To get around this problem, a practical solution has been to synthesize soluble lipid analogs and study the biosynthetic enzymes using a soluble system. This has been done for the Bacillus subtilis teichoic acid polymerase, TagF, although several aspects of catalysis were inconsistent with the results obtained with reconstituted membrane systems or physiological observations. In this work we explored the acceptor substrate promiscuity and polymer length disregulation that appear to be characteristic of TagF activity away from biological membranes. Using isotope labeling, steady-state kinetics, and chemical lability studies, we demonstrated that the enzyme can synthesize poly(glycerol phosphate) teichoic acid using the elongation substrate CDP-glycerol as an acceptor. This suggests that substrate specificity is relaxed in the region distal to the glycerol phosphate moiety in the acceptor molecule under these conditions. Polymer synthesis proceeded at a rate (27 min(-1)) comparable to that in the reconstituted membrane system after a distinct lag period which likely rep...Continue Reading

References

Feb 1, 1992·Journal of Bacteriology·S M SteenbergenE R Vimr
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J Mauck, L Glaser
Sep 30, 1980·Biochemical and Biophysical Research Communications·Y SasakiE Ito
Jun 1, 1981·Journal of Bacteriology·J LeaverJ Baddiley
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Jul 25, 1959·Nature·J J ARMSTRONGF C NEUHAUS
Dec 20, 2003·Acta Crystallographica. Section D, Biological Crystallography·Sanjan K DasDavid W Rice
Sep 6, 2005·The Journal of Biological Chemistry·Jeffrey W SchertzerEric D Brown
Oct 3, 2006·Journal of Bacteriology·Michael A D'EliaEric D Brown
Dec 14, 2006·ACS Chemical Biology·Cynthia GinsbergSuzanne Walker
May 10, 2008·Chembiochem : a European Journal of Chemical Biology·Mark P PereiraEric D Brown

❮ Previous
Next ❯

Citations

May 8, 2009·Journal of the American Chemical Society·Shasad SharifJacob Schaefer
Apr 20, 2010·Nature Structural & Molecular Biology·Andrew L LoveringNatalie C J Strynadka
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Leslie CuthbertsonChris Whitfield
Sep 13, 2013·Annual Review of Microbiology·Stephanie BrownSuzanne Walker
Oct 31, 2013·The Journal of Antibiotics·Edward W C Sewell, Eric D Brown
Nov 10, 2009·Chembiochem : a European Journal of Chemical Biology·Jonathan G SwobodaSuzanne Walker
Jun 23, 2020·Annual Review of Biochemistry·Christopher A CaffaletteJochen Zimmer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.