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Use of chelating agents as terminators of alkaline phosphatase activity in enzyme-linked immunosorbent assay (ELISA) tests

Journal of Immunological Methods

Jan 1, 1981

P Brauner, B Fridlender

PMID: 6270211

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Abstract

For a long time chelating agents have been known to be inhibitors of alkaline phosphatase activity. However, the use of chelating agents in stopping alkaline phosphatase activity in ELISA reactions is a novel application with several advantages over conventionally used acids or bases.

Mentioned in this Paper

Enzyme-Linked Immunosorbent Assay
Alkaline Phosphatase Activity
Edetic Acid, Calcium, Sodium Salt
Metal Antagonists
Acids
Cytomegalovirus Infections
Egtazic Acid Potassium Salt
Cysteine Hydrochloride
Chelating Agents
Alkaline Phosphatase
Paper Details
References

Use of chelating agents as terminators of alkaline phosphatase activity in enzyme-linked immunosorbent assay (ELISA) tests

Journal of Immunological Methods

Jan 1, 1981

P Brauner, B Fridlender

PMID: 6270211

DOI:

Abstract

For a long time chelating agents have been known to be inhibitors of alkaline phosphatase activity. However, the use of chelating agents in stopping alkaline phosphatase activity in ELISA reactions is a novel application with several advantages over conventionally used acids or bases.

Mentioned in this Paper

Enzyme-Linked Immunosorbent Assay
Alkaline Phosphatase Activity
Edetic Acid, Calcium, Sodium Salt
Metal Antagonists
Acids

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