Use of chelating agents as terminators of alkaline phosphatase activity in enzyme-linked immunosorbent assay (ELISA) tests
Journal of Immunological Methods
Jan 1, 1981
P Brauner, B Fridlender
PMID: 6270211
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Abstract
For a long time chelating agents have been known to be inhibitors of alkaline phosphatase activity. However, the use of chelating agents in stopping alkaline phosphatase activity in ELISA reactions is a novel application with several advantages over conventionally used acids or bases.
Mentioned in this Paper
Enzyme-Linked Immunosorbent Assay
Alkaline Phosphatase Activity
Edetic Acid, Calcium, Sodium Salt
Metal Antagonists
Acids
Cytomegalovirus Infections
Egtazic Acid Potassium Salt
Cysteine Hydrochloride
Chelating Agents
Alkaline Phosphatase
Paper Details
References
- References3
- Citations2
RELEASE OF ALKALINE PHOSPHATASE FROM CELLS OF ESCHERICHIA COLI UPON LYSOZYME SPHEROPLAST FORMATION
BiochemistryDecember 1, 1964M H Malamy, Bernard L HoreckerAlkaline phosphatase of Escherichia coli: a zinc metalloenzyme
BiochemistryMay 25, 1962D J PlockeBert L Vallee
Repression of alkaline phosphatase in human cell cultures by cystine and cysteine
Proceedings of the National Academy of Sciences of the United States of AmericaApril 1, 1963Rachel A Cox, Craig MacLeod
1
- References3
- Citations2
Optimal conditions for the preparation of ferritin-labeled antibodies defined by binding to their antigen in an ELISA
ImmunobiologyMay 1, 1984Ernst W RauterbergW RömerCellular enzyme-linked immunospecific assay (CELISA). IV. Inhibition of endogenous cellular alkaline phosphatase activity
Journal of Immunological MethodsMarch 30, 1984Randall E Morris, Daniel Horowitz
1
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Use of chelating agents as terminators of alkaline phosphatase activity in enzyme-linked immunosorbent assay (ELISA) tests
Abstract
For a long time chelating agents have been known to be inhibitors of alkaline phosphatase activity. However, the use of chelating agents in stopping alkaline phosphatase activity in ELISA reactions is a novel application with several advantages over conventionally used acids or bases.
Mentioned in this Paper
Enzyme-Linked Immunosorbent Assay
Alkaline Phosphatase Activity
Edetic Acid, Calcium, Sodium Salt
Metal Antagonists
Acids
Related Papers
Paper Details
References
- References3
- Citations2
RELEASE OF ALKALINE PHOSPHATASE FROM CELLS OF ESCHERICHIA COLI UPON LYSOZYME SPHEROPLAST FORMATION
BiochemistryDecember 1, 1964M H Malamy, Bernard L HoreckerAlkaline phosphatase of Escherichia coli: a zinc metalloenzyme
BiochemistryMay 25, 1962D J PlockeBert L ValleeRepression of alkaline phosphatase in human cell cultures by cystine and cysteine
Proceedings of the National Academy of Sciences of the United States of AmericaApril 1, 1963Rachel A Cox, Craig MacLeod
1
- References3
- Citations2
Optimal conditions for the preparation of ferritin-labeled antibodies defined by binding to their antigen in an ELISA
ImmunobiologyMay 1, 1984Ernst W RauterbergW RömerCellular enzyme-linked immunospecific assay (CELISA). IV. Inhibition of endogenous cellular alkaline phosphatase activity
Journal of Immunological MethodsMarch 30, 1984Randall E Morris, Daniel Horowitz
1
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