Use of chitosan conduit combined with bone marrow mesenchymal stem cells for promoting peripheral nerve regeneration.

Journal of Materials Science. Materials in Medicine
Lei Zheng, Hui-Fei Cui

Abstract

Many studies have been dedicated to the development of scaffolds for improving post-traumatic nerve regeneration. The goal of this study was to develop and test chitosan conduit to use in peripheral nerve reconstruction, either alone or combined with bone marrow mesenchymal stem cells (BMSCs). In this study, the roles of the degree of deacetylation (DD) and molecular weight of chitosan on some biological properties of chitosan films, including hydrophilicity, degradation and BMSCs affinity were investigated. The molecular weight of Chitosans used are 5 x 10(4) Da, 2 x 10(5) Da, 5 x 10(5) Da, 1 x 10(6) Da, the deacetylation degrees are 85, 95%, respectively. The affinity of eight kinds of Chitosans to the BMSCs was assessed by MTT assay, the contact angle and the degradation time of the materials in vivo were also measured. Chitosans with the molecular weight of 1 x 10(6) Da and DD of 95% can significantly promote the survival and outgrowth of cells, which have better hydrophilicity and can remain integrity even after 8 to 16 weeks, all of above meet the requirement of nerve engineering. The BMSCs we transplanted can differentiate into neural stem cells in vivo, and the materials we selected combined with BMSCs can bridge 8-mm-l...Continue Reading

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Citations

Jun 5, 2012·Journal of Materials Science. Materials in Medicine·Lei Zheng, Hui-Fei Cui
Sep 16, 2011·Regenerative Medicine·Edward C TollMartin A Birchall
May 13, 2014·Journal of Neurosurgery·Caroline A HundepoolSteven E R Hovius
Jul 31, 2013·Hand Clinics·Michael Y LinRanjan Gupta
Feb 7, 2012·Progress in Neurobiology·Mina MekhailMaryam Tabrizian
Mar 27, 2015·Journal of Biomedical Materials Research. Part a·Rao JianGao Yilu
Mar 29, 2014·Journal of Biomedical Materials Research. Part a·Mohammad-Hossein BeigiMohammad-Hossein Nasr-Esfahani
Oct 23, 2012·Journal of Tissue Engineering and Regenerative Medicine·Sook Young ParkHyun Jeong Kim
May 9, 2012·International Journal of Biological Macromolecules·Jixiang ZhuJun Wan
Feb 16, 2012·Biochemical and Biophysical Research Communications·Takuya UemuraHiroaki Nakamura
Feb 19, 2016·Biomaterials Science·Stephanie KnowltonSavas Tasoglu
Apr 29, 2014·World Journal of Stem Cells·Ana Maria Blanco MartinezFernanda Martins Almeida
Mar 16, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhe Zhang, Huifei Cui
Feb 26, 2019·Neural Regeneration Research·Muhammad Sana Ullah SaharGeoffrey Douglas Tansley
Jul 17, 2018·BioMed Research International·Giulia RonchiGiovanna Gambarotta
Jan 11, 2019·Frontiers in Cellular Neuroscience·Jesús Chato-AstrainVíctor Carriel
Mar 6, 2021·Neurochemistry International·Santiago Grijalvo, David Díaz Díaz
Apr 6, 2021·Neural Regeneration Research·Rui-Cheng ZhangDe-Qin Geng
Aug 18, 2020·Plastic and Reconstructive Surgery. Global Open·Riccardo SchweizerVijay S Gorantla
Apr 16, 2018·Advanced Healthcare Materials·Metin UzSurya K Mallapragada

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