PMID: 6971154Jan 1, 1981Paper

Use of cloned populations of mouse lymphocytes to analyze cellular differentiation

Cell
G NabelH Cantor

Abstract

We describe a method for generation of homogeneous cell populations that each arise from clonal expansion of cells at a discrete stage of differentiation within a single lineage. We have used this to produce continuously propagatable lymphocyte clones. Each clone represents a cell at a progressive stage of thymus-dependent cellular differentiation. These cloned cells bear stable surface membrane glycoproteins characteristic of precursor cells and mature progeny; conditions allowing maximal cloning efficiencies for each cell type (10-85%) have been established. Mature lymphocyte clones continue to express specialized function and provide material for biochemical analysis of T lymphocyte functions; one fully differentiated clone from the "inducer" lymphocyte set synthesizes a molecule that activates other lymphocytes to secrete immunoglobulin. This activity is associated with a highly purified molecule having a molecular weight of 45,000 daltons and an isoelectric point of approximately 6.0. This molecule, together with clones of precursor and mature T lymphocytes, may provide a system to further study the mechanisms of gene activation during cellular differentiation.

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Citations

Jan 1, 1982·Biophysics of Structure and Mechanism·J H Peters, W J Müller-Hermes
Jul 30, 1982·Journal of Immunological Methods·W Haas, H Von Boehmer
Jan 1, 1982·Journal of Immunological Methods·B Sredni
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Oct 1, 1996·Cytokine & Growth Factor Reviews·G F Weber, H Cantor
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G NabelH Cantor
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G J FreemanH Cantor
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T YokotaK Arai
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Jun 1, 1981·The Journal of Experimental Medicine·G NabelH Cantor
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Aug 1, 1982·The Journal of Experimental Medicine·G NabelH Cantor
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