Use of colicin e3 for biological containment of microorganisms.

Applied and Environmental Microbiology
M MunthaliE Díaz

Abstract

The genetic determinant of the lethal antibiotic colicin E3 was cloned under the control of a tightly regulated promoter in the absence of the gene for its cognate inhibitor. Combination of this killing cassette with a stringent regulatory element provided a substrate-dependent conditional suicide system that was exploited for the biological containment of a Pseudomonas putida strain. The lethality of a single gene copy and the distinct and universal cellular target of the antibiotic suggest colicin E3 as an ideal candidate for combination with other lethal functions to design highly efficient containment systems for microorganisms.

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Citations

Jan 25, 2011·Microbial Biotechnology·Jens KrollAlexander Steinbüchel
Dec 10, 2003·Microbiology·Begoña TorresEduardo Díaz
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
May 18, 2011·Antimicrobial Agents and Chemotherapy·Fernando BaqueroFernando de la Cruz
Mar 9, 1999·Folia Microbiologica·J Smarda, D Smajs
Jun 25, 2015·Microbiology Spectrum·José L GarcíA, Eduardo Díaz
Jun 25, 2015·Microbiology Spectrum·Esteban Martínez-GarcíaVíctor De Lorenzo

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