PMID: 1208431Nov 1, 1975Paper

Use of colour reactions to identify lipid classes, including tocopherols, by thin-layer chromatography

Prikladnaia biokhimiia i mikrobiologiia
L I Semyonova, Kuznetsov

Abstract

Colour reactions of different classes and individual lipids of plant origin with organic and inorganic compounds (used as reagents) were studied to identify them in thin-layer silica gel. Some of the reagents (p-dimethyl phenylene diamine, chromotropic acid in H2SO4, Ti(SO4)2 in H2SO4) helped to detect main lipid classes and to identify selectively individual compounds (alpha- and gamma-tocopherols). Hydrochloric p-nitrophenyl diazonium, p-dimethyl aminobenzaldehyde are of interest mainly as group reagents to lipid classes with common properties. The reaction sensitivity is measured in micrograms.

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