Use of combined detection of hepatitis C virus core antigen and antibodies to reduce the serological window-phase

Pathologie-biologie
F HmaïedS Ben Redjeb

Abstract

In this study, we aimed at evaluating the performances of a combined assay for the detection of hepatitis C virus core antigen and antibodies and comparing this test with conventional third generation Elisa. Two hundred forty-one samples were included in this study and tested by Monolisa HCV Ag-Ab ULTRA, Biorad and compared to Monolisa Anti-HCV Plus. A comparative study was performed on a HCV seroconversion panel (Monolisa anti-HCV Plus, Biorad; Innotest HCV Ab IV, Innogenetics and Murex anti-HCV, Abbott). False positive samples were detected with western blot assay (INNO-LIA HCV Ab III, Innogenetics). Two anti-HCV negative haemodialysis patients with rise in ALT have been tested for RNA detection (Amplicor v2.0, Roche). Results obtained with Biorad Ag-Ab were in agreement with third generation ELISA on HCV seroconversion panel. From anti-HCV negative patients, four samples were found low positive with HCV Ag-Ab. Two anti-HCV negative haemodialysis patients/HCV RNA positive were also negative with HCV Ag-Ab and 13 low positive samples with Biorad Ab were found negative with Ag-Ab. The HCV Ag-Ab assay has a high specificity and sensitivity comparatively to conventional ELISA; but in our study we don't prove the reduction of the ...Continue Reading

References

Aug 1, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E TanakaH J Alter
Jul 11, 2001·Journal of Virological Methods·J B KurtzC Chandler
Jan 17, 2002·The New England Journal of Medicine·D J Wyler
Apr 9, 2004·Journal of Clinical Microbiology·Dale M NetskiJohn R Ticehurst
May 26, 2004·Journal of Virological Methods·Laurence CagnonKenneth A Simmen
Jul 1, 2004·Transfusion·Magdalena LetowskaAleksandra Rosiek
Jan 18, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Katja SemeAdriana Vince

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