Use of contraceptive depot medroxyprogesterone acetate is associated with impaired cervicovaginal mucosal integrity

The Journal of Clinical Investigation
Irina A ZalenskayaGustavo F Doncel

Abstract

Injectable depot medroxyprogesterone acetate (DMPA) is one of the most popular contraception methods in areas of high HIV seroprevalence. Evidence is accumulating that use of DMPA might be associated with an increased risk of HIV-1 acquisition by women; however, mechanisms of this association are not completely understood. The goal of this study was to gain insight into mechanisms underlying the possible link between use of DMPA and risk of HIV-1 acquisition, exploring transcription profiling of ectocervical tissues. Healthy women received either DMPA (n = 31) or combined oral contraceptive (COC), which has not been linked to an increased risk of HIV acquisition (n = 32). We conducted a comparative microarray-based whole-genome transcriptome profiling of human ectocervical tissues before and after 6 weeks of hormonal contraception use. The analysis identified that expression of 235 and 76 genes was significantly altered after DMPA and COC use, respectively. The most striking effect of DMPA, but not COC, was significantly altered expression (mostly downregulation) of many genes strategically involved in the maintenance of mucosal barrier function; the alterations, as indicated by Ingenuity Pathway Analysis (IPA), were most likel...Continue Reading

References

Jan 1, 1991·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C A Squier
Feb 1, 1991·The Journal of Infectious Diseases·F A PlummerA R Ronald
Oct 1, 1986·Journal of the American Academy of Dermatology·P M Elias
Sep 1, 1989·Molecular Endocrinology·I E AlexanderR L Sutherland
Jan 1, 1966·The Anatomical Record·T E Barker, B E Walker
Apr 1, 1973·Fertility and Sterility·R C Jones, R A Edgren
May 1, 1974·Experimental Cell Research·S H Yuspa, C C Harris
May 1, 1994·Differentiation; Research in Biological Diversity·I B Gimenez-ContiC J Conti
Aug 19, 2000·The Journal of Infectious Diseases·S M SmithP A Marx
Aug 29, 2000·Obstetrics and Gynecology·L MillerD A Eschenbach
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·K J Green, C A Gaudry
Jun 1, 2001·Fertility and Sterility·F WieserJ C Huber
Mar 26, 2004·Nature Reviews. Microbiology·Robin J Shattock, John P Moore
Jan 6, 2005·The Journal of Infectious Diseases·Khalil G GhanemUNKNOWN HIV Epidemiology Research Study Group
Feb 8, 2005·Biochemical and Biophysical Research Communications·Sheri L HolmenBart O Williams
Apr 2, 2005·Nature Reviews. Molecular Cell Biology·Eleonora CandiGerry Melino
Feb 24, 2006·Journal of Cell Science·Margaret D KottkeAndrew P Kowalczyk
Dec 2, 2008·Experimental Dermatology·Ehrhardt ProkschJens-Michael Jensen
Feb 13, 2009·Best Practice & Research. Clinical Obstetrics & Gynaecology·Charles S MorrisonLaShawn B Jones
May 9, 2009·Journal of Anatomy·Hermann H Bragulla, Dominique G Homberger
Sep 14, 2010·American Journal of Obstetrics and Gynecology·Iara M LinharesSteven S Witkin
Nov 26, 2010·Journal of Leukocyte Biology·George B SegelMarshall A Lichtman
Apr 8, 2011·Biology of Reproduction·Caitlin D BlaskewiczDeborah J Anderson
Apr 23, 2011·Molecular Endocrinology·Jamie R Flammer, Inez Rogatsky
Jun 23, 2011·Molecular and Cellular Endocrinology·Atul PurohitBarry V L Potter

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Citations

Jun 17, 2020·Biology of Reproduction·Nirk E Quispe CallaThomas L Cherpes
Apr 19, 2019·AIDS Research and Human Retroviruses·Andrea ThurmanGustavo F Doncel
Dec 24, 2018·Journal of Women's Health·Erica L GollubZena Stein
Mar 1, 2021·American Journal of Reproductive Immunology : AJRI·Refilwe P MolatlhegiLyle R McKinnon
Apr 30, 2021·Acta Biomaterialia·Anna StejskalováBenjamin D Almquist
May 16, 2021·American Journal of Reproductive Immunology : AJRI·Hossaena AyeleAdam Burgener
Jun 3, 2021·American Journal of Reproductive Immunology : AJRI·Janine JewanrajLenine J P Liebenberg

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Datasets Mentioned

BETA
GSE110313

Methods Mentioned

BETA
contraception
PCR
biopsies
light microscopy

Clinical Trials Mentioned

NCT01421368

Software Mentioned

Upstream Regulator Analysis tool
Ingenuity Pathway Analysis ( IPA )
ArrayTools
SAS
IPA
GraphPad Prism
Affymetrix ) Arrays Tool

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