Use of controlled luciferase expression to monitor chemicals affecting protein synthesis.

Applied and Environmental Microbiology
J LampinenM Karp

Abstract

In this article, we present a new bioluminescent test system for the screening of chemical compounds with an inhibitory effect on protein synthesis. The test is based on the measurement of real-time in vivo light production by Escherichia coli strains expressing different luciferase genes. The eukaryotic lucGR gene from Pyrophorus plagiophthalamus was found to be the best of three types of luciferase genes tested. Chemicals with known inhibitory effects on protein synthesis were used as test chemicals together with some general toxicants. The incubation of a test chemical with cells was performed either prior to or after the induction of protein synthesis, and the difference in the results of the two methods distinguishes the possible influence on protein synthesis from direct metabolic inhibition. Using lyophilized bacteria, the test is performed in less than an hour without any bacterial cultivation, which makes the test suitable for rapid and sensitive screening of chemicals or environmental samples. Compared with the standardized 50% inhibitory concentration calculation method of the bioluminescent cytotoxicity test, the more direct approach of calculation developed in this study proved to be more convenient than and as rel...Continue Reading

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Citations

Nov 16, 2001·Antimicrobial Agents and Chemotherapy·M TenhamiM Karp
May 15, 2002·Journal of Biomolecular Screening·Satu J ValtonenMatti T Karp
Jul 15, 2003·Journal of Biomolecular Screening·Lorenzo Galluzzi, Matti Karp
Mar 10, 2019·Current Genetics·Aubin Fleiss, Karen S Sarkisyan
May 15, 2002·Journal of Biomolecular Screening·Minna-Liisa AnköMatti Karp
Jun 28, 2016·Antibiotics·Ilya A OstermanPetr V Sergiev
Feb 18, 2011·Bioengineered Bugs·Chun LiKlara Stensvåg
Jul 2, 1998·Applied and Environmental Microbiology·G CorthierP Renault
Dec 5, 1998·Biosensors & Bioelectronics·S TauriainenM Virta
Sep 5, 2002·Biochemical and Biophysical Research Communications·Annette Westerlund-KarlssonMatti Karp
Aug 24, 2000·Journal of Agricultural and Food Chemistry·J KurittuM Karp

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